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Literature summary for 4.2.1.10 extracted from

  • Evans, L.D.B.; Roszak, A.W.; Noble, L.J.; Robinson, D.A.; Chalk, P.A.; Matthews, J.L.; Coggins, J.R.; Price, N.C.; Lapthorn, A.J.
    Specificity of substrate recognition by type II dehydroquinases as revealed by binding of polyanions (2002), FEBS Lett., 530, 24-30.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Streptomyces coelicolor
expressed in Escherichia coli Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
phosphate
-
Streptomyces coelicolor
sulfate
-
Streptomyces coelicolor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.024
-
3-dehydroquinate pH 7, 25°C Mycobacterium tuberculosis
0.1
-
3-dehydroquinate pH 7, 25°C Streptomyces coelicolor

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WPX7
-
-
Mycobacterium tuberculosis H37Rv P9WPX7
-
-
Streptomyces coelicolor
-
-
-

Reaction

Reaction Comment Organism Reaction ID
3-dehydroquinate = 3-dehydroshikimate + H2O type I and type II DHQases are quite distinct from one another in terms of amino acid sequences and three-dimensional structures. Type I enzymes involve a syn elimination and catalyse the biosynthetic reaction. By contrast type II enzymes, which can catalyse either the biosynthetic or catabolic reactions, involve and anti elimination reaction Streptomyces coelicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-dehydroquinate
-
Streptomyces coelicolor 3-dehydroshikimate + H2O
-
?
3-dehydroquinate
-
Mycobacterium tuberculosis 3-dehydroshikimate + H2O
-
?
3-dehydroquinate
-
Mycobacterium tuberculosis H37Rv 3-dehydroshikimate + H2O
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.2
-
3-dehydroquinate pH 7, 25°C Mycobacterium tuberculosis
124
-
3-dehydroquinate pH 7, 25°C Streptomyces coelicolor

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
7
-
phosphate pH 7, 25°C Streptomyces coelicolor
11
-
sulfate pH 7, 25°C Streptomyces coelicolor