Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Streptomyces coelicolor |
expressed in Escherichia coli | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phosphate | - |
Streptomyces coelicolor | |
sulfate | - |
Streptomyces coelicolor |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
3-dehydroquinate | pH 7, 25°C | Mycobacterium tuberculosis | |
0.1 | - |
3-dehydroquinate | pH 7, 25°C | Streptomyces coelicolor |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WPX7 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WPX7 | - |
- |
Streptomyces coelicolor | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
3-dehydroquinate = 3-dehydroshikimate + H2O | type I and type II DHQases are quite distinct from one another in terms of amino acid sequences and three-dimensional structures. Type I enzymes involve a syn elimination and catalyse the biosynthetic reaction. By contrast type II enzymes, which can catalyse either the biosynthetic or catabolic reactions, involve and anti elimination reaction | Streptomyces coelicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-dehydroquinate | - |
Streptomyces coelicolor | 3-dehydroshikimate + H2O | - |
? | |
3-dehydroquinate | - |
Mycobacterium tuberculosis | 3-dehydroshikimate + H2O | - |
? | |
3-dehydroquinate | - |
Mycobacterium tuberculosis H37Rv | 3-dehydroshikimate + H2O | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.2 | - |
3-dehydroquinate | pH 7, 25°C | Mycobacterium tuberculosis | |
124 | - |
3-dehydroquinate | pH 7, 25°C | Streptomyces coelicolor |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
7 | - |
phosphate | pH 7, 25°C | Streptomyces coelicolor | |
11 | - |
sulfate | pH 7, 25°C | Streptomyces coelicolor |