Any feedback?
Literature summary for 4.2.1.1 extracted from
- Characterization of two novel nodule-enhanced alpha-type carbonic anhydrases from Lotus japonicus (2011), Biochim. Biophys. Acta, 1814, 496-504.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| isozyme LjCAA1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, expression analysis by quantitative real-time RT-PCR, and spatial expression profiling, recombinant expression of His-tagged isozyme in Escherichia coli strain BL21(DE3) | Lotus japonicus |
| isozyme LjCAA2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, expression analysis by quantitative real-time RT-PCR, and spatial expression profiling, recombinant expression of His-tagged isozymes in Escherichia coli strain BL21(DE3) | Lotus japonicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics, overview | Lotus japonicus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | required, binding structure modelling, overview | Lotus japonicus |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lotus japonicus | A7DX12 | isozyme LjCAA1; isozymes LjCAA1 and LjCAA2 | - |
| Lotus japonicus | A7DX13 | isozyme LjCAA2; isozymes LjCAA1 and LjCAA2 | - |
| Lotus japonicus Gifu B-129 | A7DX12 | isozyme LjCAA1; isozymes LjCAA1 and LjCAA2 | - |
| Lotus japonicus Gifu B-129 | A7DX13 | isozyme LjCAA2; isozymes LjCAA1 and LjCAA2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged isozyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Lotus japonicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | expression patterns of isozymes LjCAA1 and LjCAA2 by in situ hybridization, both are expressed in nodule inner cortical cells, vascular bundles and central tissue, overview | Lotus japonicus | - |
| root nodule | inner cortical cells, both genes encoding LjCAA1 and LjCAA2 code for nodule enhanced carbonic anhydrase isoforms, which are induced early during nodule development, overview | Lotus japonicus | - |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 30294.62, isozyme LjCAA1, sequence calculation | Lotus japonicus |
| ? | x * 30740.77, isozyme LjCAA2, sequence calculation | Lotus japonicus |
| More | molecular modeling, overview | Lotus japonicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-type carbonic anhydrase | - |
Lotus japonicus |
| CAA1 | - |
Lotus japonicus |
| CAA2 | - |
Lotus japonicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | - |
assay at | Lotus japonicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.3 | - |
bicarbonate dehydration assay at | Lotus japonicus |
| 7.5 | - |
CO2 hydration assay at | Lotus japonicus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Lotus japonicus | isozyme LjCAA2, sequence calculation | - |
6.63 |
| Lotus japonicus | isozyme LjCAA1, sequence calculation | - |
9.05 |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Lotus japonicus | the gene encoding isozyme LjCAA1 is slightly to moderately down-regulated in ineffective nodules formed by mutant Mesorhizobium loti strains | down |
| Lotus japonicus | the gene encoding isozyme LjCAA2 is slightly to moderately down-regulated in ineffective nodules formed by mutant Mesorhizobium loti strains | down |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | isozyme LjCAA1 is involved in nitrogen ficxation/assimilation, but may also be involved in biochemical and physiological processes not directly linked to nitrogen fixation/assimilation | Lotus japonicus |
| physiological function | isozyme LjCAA2 is involved in nitrogen fixation/assimilation, but may also be involved in biochemical and physiological processes not directly linked to nitrogen fixation/assimilation | Lotus japonicus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
