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Literature summary for 4.2.1.1 extracted from

  • Kalloniati, C.; Tsikou, D.; Lampiri, V.; Fotelli, M.N.; Rennenberg, H.; Chatzipavlidis, I.; Fasseas, C.; Katinakis, P.; Flemetakis, E.
    Characterization of a Mesorhizobium loti alpha-type carbonic anhydrase and its role in symbiotic nitrogen fixation (2009), J. Bacteriol., 191, 2593-2600.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Mesorhizobium loti

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
31.73
-
 CO2 recombinant enzyme, at 4°C in a HEPES/NaOH, pH 7.5 Mesorhizobium loti
112.7
-
 H2CO3 recombinant enzyme, at 4°C in a MES/KOH pH 6.3 buffer Mesorhizobium loti

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ zinc-containing metalloenzyme Mesorhizobium loti

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 SDS-PAGE Mesorhizobium loti
27200
-
 calculated from amino acid sequence Mesorhizobium loti

Organism

Organism UniProt Comment Textmining
Mesorhizobium loti Q8KJM6
-
-
Mesorhizobium loti R7A Q8KJM6
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity column chromatography Mesorhizobium loti

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
285000
-
 reverse reaction of bicarbonate dehydration at 4°C in a MES/KOH pH 6.3 buffer Mesorhizobium loti
1914000
-
 forward reaction of CO2 dehydration at 4°C in a HEPES/NaOH, pH 7.5 Mesorhizobium loti

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CO2 + H2O
-
 Mesorhizobium loti H2CO3
-
 r
CO2 + H2O
-
 Mesorhizobium loti R7A H2CO3
-
 r

Synonyms

Synonyms Comment Organism
alpha-type carbonic anhydrase
-
 Mesorhizobium loti
CAA1
-
 Mesorhizobium loti

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
121500
-
 H2CO3 recombinant enzyme, at 4°C in a MES/KOH pH 6.3 buffer Mesorhizobium loti
813600
-
 CO2 recombinant enzyme, at 4°C in a HEPES/NaOH, pH 7.5 Mesorhizobium loti

Expression

Organism Comment Expression
Mesorhizobium loti gene expression is induced by increased medium pH up