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Literature summary for 4.2.1.1 extracted from

  • Soyut, H.; Beydemir, S.; Hisar, O.
    Effects of some metals on carbonic anhydrase from brains of rainbow trout (2008), Biol. Trace Elem. Res., 123, 179-190.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Ag+ silver inhibits competitively Oncorhynchus mykiss
Cd2+ cadmium inhibits competitively Oncorhynchus mykiss
Co2+ cobalt inhibits competitively Oncorhynchus mykiss
Cu2+ copper inhibits noncompetitively Oncorhynchus mykiss
Zn2+ zinc inhibits uncompetitively Oncorhynchus mykiss

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27300
-
native enzyme, estimated by gel filtration Oncorhynchus mykiss
29000
-
subunit, SDS-PAGE Oncorhynchus mykiss

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CO2 + H2O Oncorhynchus mykiss
-
H2CO3
-
r

Organism

Organism UniProt Comment Textmining
Oncorhynchus mykiss
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Sepharose-4B-L-tyrosine-sulfanilamide affinity chromatography Oncorhynchus mykiss

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Oncorhynchus mykiss
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
enzyme from lysate shows a specific activity of 1.77 EU/mg, the 1280fold purified enzyme shows a specific activity of 2275 EU/mg Oncorhynchus mykiss

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CO2 + H2O
-
Oncorhynchus mykiss H2CO3
-
r

Synonyms

Synonyms Comment Organism
carbonate hydrolyase
-
Oncorhynchus mykiss
carbonic anhydrase
-
Oncorhynchus mykiss

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
in 1 M Tris-SO4 at 4°C Oncorhynchus mykiss

pH Stability

pH Stability pH Stability Maximum Comment Organism
9
-
stable at pH 9.0 in 1 M Tris-SO4 at 4°C Oncorhynchus mykiss

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.014
-
Co2+ in 0.05 M Tris-SO4 buffer, at pH 9.0 and 25°C Oncorhynchus mykiss
2.15
-
Zn2+ in 0.05 M Tris-SO4 buffer, at pH 9.0 and 25°C Oncorhynchus mykiss
27.68
-
Cu2+ in 0.05 M Tris-SO4 buffer, at pH 9.0 and 25°C Oncorhynchus mykiss
94.18
-
Cd2+ in 0.05 M Tris-SO4 buffer, at pH 9.0 and 25°C Oncorhynchus mykiss
193.9
-
Ag+ in 0.05 M Tris-SO4 buffer, at pH 9.0 and 25°C Oncorhynchus mykiss

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.05
-
in 0.05 M Tris-SO4 buffer, at pH 9.0 and 25°C Oncorhynchus mykiss Co2+
0.31
-
in 0.05 M Tris-SO4 buffer, at pH 9.0 and 25°C Oncorhynchus mykiss Zn2+
30
-
in 0.05 M Tris-SO4 buffer, at pH 9.0 and 25°C Oncorhynchus mykiss Cu2+
82.5
-
in 0.05 M Tris-SO4 buffer, at pH 9.0 and 25°C Oncorhynchus mykiss Cd2+
159
-
in 0.05 M Tris-SO4 buffer, at pH 9.0 and 25°C Oncorhynchus mykiss Ag+