Literature summary for 4.2.1.1 extracted from

Premkumar, L.; Bageshwar, U.K.; Gokhman, I.; Zamir, A.; Sussman, J.L.
An unusual halotolerant alpha-type carbonic anhydrase from the alga Dunaliella salina functionally expressed in Escherichia coli (2003), Protein Expr. Purif., 28, 151-157.

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Cloned(Commentary)

Cloned (Comment)	Organism
functionally expressed in Escherichia coli Origami B(DE3)pLys	Dunaliella salina

General Stability

General Stability	Organism
presence of salt, 0-4 M NaCl, during Dca storage has no effect on the enzyme stability and activity	Dunaliella salina

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
plasma membrane	-	Dunaliella salina	5886	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
60000	-	Western blot analysis, SDS-PAGE	Dunaliella salina

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
H2CO3	Dunaliella salina	-	CO2 + H2O	-	r

Organism

Organism	UniProt	Comment	Textmining
Dunaliella salina	P54212	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme	Dunaliella salina

Source Tissue

Source Tissue	Comment	Organism	Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	specific activity 393 U/mg, enzyme units are defined as Wilbur-Anderson (WA) units	Dunaliella salina
0.161	-	-	Dunaliella salina

Storage Stability

Storage Stability	Organism
-20°C, Tris-SO4, pH 8.8, purified enzyme, solution remains fully soluble and active for at least 2 months	Dunaliella salina
-80°C, recombinant bacterial cell pellets can be stored for several months without apparent loss in enzyme activity	Dunaliella salina
4°C, Tris-SO4, pH 8.8, purified enzyme, solution remains fully soluble and active for at least 2 months	Dunaliella salina

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl acetate + H2O	-	Dunaliella salina	4-nitrophenol + acetate	-	r
H2CO3	-	Dunaliella salina	CO2 + H2O	-	r

Synonyms

Synonyms	Comment	Organism
alpha-type carbonic anhydrase	-	Dunaliella salina
Dca	-	Dunaliella salina

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Release 2023.1 (January 2023)