Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) or Bl21(DE3) Gold | Methanosarcina thermophila |
Protein Variants | Comment | Organism |
---|---|---|
E62A | site-directed mutagenesis, large decrease in kcat | Methanosarcina thermophila |
E62A/E84A | site-directed mutagenesis, large decrease in kcat | Methanosarcina thermophila |
E62C | site-directed mutagenesis, large decrease in kcat | Methanosarcina thermophila |
E62D | site-directed mutagenesis, 3fold decrease in kcat | Methanosarcina thermophila |
E62D/E84D | site-directed mutagenesis, large decrease in kcat | Methanosarcina thermophila |
E62H | site-directed mutagenesis, large decrease in kcat | Methanosarcina thermophila |
E62Q | site-directed mutagenesis, large decrease in kcat | Methanosarcina thermophila |
E62T | site-directed mutagenesis, large decrease in kcat | Methanosarcina thermophila |
E62Y | site-directed mutagenesis, large decrease in kcat | Methanosarcina thermophila |
E84 A | site-directed mutagenesis, large decrease in kcat values in pH 7.5 MOPS buffer | Methanosarcina thermophila |
E84 C | site-directed mutagenesis, large decrease in kcat values in pH 7.5 MOPS buffer | Methanosarcina thermophila |
E84 D | site-directed mutagenesis, large decrease in kcat values in pH 7.5 MOPS buffer | Methanosarcina thermophila |
E84 H | site-directed mutagenesis, large decrease in kcat values in pH 7.5 MOPS buffer | Methanosarcina thermophila |
E84 K | site-directed mutagenesis, large decrease in kcat values in pH 7.5 MOPS buffer | Methanosarcina thermophila |
E84 Q | site-directed mutagenesis, large decrease in kcat values in pH 7.5 MOPS buffer | Methanosarcina thermophila |
E84 S | site-directed mutagenesis, large decrease in kcat values in pH 7.5 MOPS buffer | Methanosarcina thermophila |
E84 Y | site-directed mutagenesis, large decrease in kcat values in pH 7.5 MOPS buffer | Methanosarcina thermophila |
E88A | site-directed mutagenesis, large decrease in kcat | Methanosarcina thermophila |
E89A | site-directed mutagenesis, large decrease in kcat | Methanosarcina thermophila |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
CO2 | pH 7.5, 25°C, variant E84A, 50 mM MOPS-Ac buffer | Methanosarcina thermophila | |
1.1 | - |
CO2 | pH 7.5, 25°C, variant E84C, 50 mM MOPS buffer | Methanosarcina thermophila | |
1.2 | - |
CO2 | pH 7.5, 25°C, variant E84Q, 50 mM MOPS buffer | Methanosarcina thermophila | |
1.4 | - |
CO2 | pH 7.5, 25°C, variant E62Q, 50 mM MOPS buffer | Methanosarcina thermophila | |
1.5 | - |
CO2 | pH 7.5, 25°C, variant E84A and E84K, 50 mM MOPS buffer | Methanosarcina thermophila | |
2.1 | - |
CO2 | pH 7.5, 25°C, variant E84S and E84Y, 50 mM MOPS buffer | Methanosarcina thermophila | |
2.5 | - |
CO2 | pH 7.5, 25°C, variant E62T, 50 mM MOPS buffer | Methanosarcina thermophila | |
2.9 | - |
CO2 | pH 7.5, 25°C, variant E62A/E84A, 50 mM MOPS buffer | Methanosarcina thermophila | |
3 | - |
CO2 | pH 7.5, 25°C, variant E62Y, 50 mM MOPS buffer | Methanosarcina thermophila | |
3.2 | - |
CO2 | pH 7.5, 25°C, variant E84H, 50 mM MOPS buffer | Methanosarcina thermophila | |
3.8 | - |
CO2 | pH 7.5, 25°C, variant E62C, 50 mM MOPS buffer | Methanosarcina thermophila | |
5.4 | - |
CO2 | pH 7.5, 25°C, variant E62D/E84D, 50 mM MOPS buffer | Methanosarcina thermophila | |
5.5 | - |
CO2 | pH 7.5, 25°C, variant E62A, 50 mM MOPS-Ac buffer | Methanosarcina thermophila | |
6.5 | - |
CO2 | pH 7.5, 25°C, variant E62A, 50 mM MOPS buffer | Methanosarcina thermophila | |
7.6 | - |
CO2 | pH 7.5, 25°C, variant E62H, 50 mM MOPS buffer | Methanosarcina thermophila | |
8.8 | - |
CO2 | pH 7.5, 25°C, variant E84A, 50 mM IMID buffer | Methanosarcina thermophila | |
9.4 | - |
CO2 | pH 7.5, 25°C, variant E62A, 50 mM IMID buffer | Methanosarcina thermophila | |
12.6 | - |
CO2 | pH 8.5, 25°C, wild-type, D2O | Methanosarcina thermophila | |
14.7 | - |
CO2 | pH 7.5, 25°C, wild-type, D2O | Methanosarcina thermophila | |
16.9 | - |
CO2 | pH 7.5, 25°C, variant E88A, 50 mM MOPS buffer | Methanosarcina thermophila | |
18.1 | - |
CO2 | pH 7.5, 25°C, variant E84D, 50 mM MOPS buffer | Methanosarcina thermophila | |
18.8 | - |
CO2 | pH 7.5, 25°C, variant E62D, 50 mM MOPS buffer | Methanosarcina thermophila | |
19.3 | - |
CO2 | pH 7.5, 25°C, variant E89A, 50 mM MOPS buffer | Methanosarcina thermophila | |
20 | - |
CO2 | pH 8.5, 25°C, wild-type, H2O | Methanosarcina thermophila | |
22.5 | - |
CO2 | pH 7.5, 25°C, wild-type, 50 mM MOPS-Ac buffer | Methanosarcina thermophila | |
23.8 | - |
CO2 | pH 6.5, 25°C, wild-type, D2O | Methanosarcina thermophila | |
27.8 | - |
CO2 | pH 7.5, 25°C, wild-type, 50 mM MOPS buffer | Methanosarcina thermophila | |
28.5 | - |
CO2 | pH 6.5, 25°C, wild-type | Methanosarcina thermophila | |
44.1 | - |
CO2 | pH 7.5, 25°C, wild-type, 50 mM IMID buffer | Methanosarcina thermophila |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | - |
Methanosarcina thermophila |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22873 | - |
3 * 22873, determined by measuring A280S of protein solution | Methanosarcina thermophila |
70000 | - |
gel filtration | Methanosarcina thermophila |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2CO3 | Methanosarcina thermophila | - |
CO2 + H2O | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina thermophila | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanosarcina thermophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2CO3 | - |
Methanosarcina thermophila | CO2 + H2O | - |
r |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 22873, determined by measuring A280S of protein solution | Methanosarcina thermophila |
Synonyms | Comment | Organism |
---|---|---|
Cam | - |
Methanosarcina thermophila |
carbonic anhydrase | - |
Methanosarcina thermophila |
gamma-class carbonic anhydrase | - |
Methanosarcina thermophila |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
47 | - |
CO2 | pH 7.5, 25°C, variant E62Y, 50 mM MOPS buffer | Methanosarcina thermophila | |
210 | - |
CO2 | pH 7.5, 25°C, variant E62A/E84A, 50 mM MOPS buffer | Methanosarcina thermophila | |
340 | - |
CO2 | pH 7.5, 25°C, variant E62A, 50 mM MOPS-Ac buffer | Methanosarcina thermophila | |
410 | - |
CO2 | pH 7.5, 25°C, variant E62Q, 50 mM MOPS buffer | Methanosarcina thermophila | |
510 | - |
CO2 | pH 7.5, 25°C, variant E84Y, 50 mM MOPS buffer | Methanosarcina thermophila | |
720 | - |
CO2 | pH 7.5, 25°C, variant E84C and E62A, 50 mM MOPS buffer | Methanosarcina thermophila | |
800 | - |
CO2 | pH 7.5, 25°C, variant E84A, 50 mM MOPS-Ac buffer | Methanosarcina thermophila | |
860 | - |
CO2 | pH 7.5, 25°C, variant E62T, 50 mM MOPS buffer | Methanosarcina thermophila | |
870 | - |
CO2 | pH 7.5, 25°C, variant E84A, 50 mM MOPS buffer | Methanosarcina thermophila | |
910 | - |
CO2 | pH 7.5, 25°C, variant E62C, 50 mM MOPS buffer | Methanosarcina thermophila | |
1050 | - |
CO2 | pH 7.5, 25°C, variant E62H, 50 mM MOPS buffer | Methanosarcina thermophila | |
1070 | - |
CO2 | pH 7.5, 25°C, variant E62A, 50 mM IMID buffer | Methanosarcina thermophila | |
1080 | - |
CO2 | pH 7.5, 25°C, variant E84S, 50 mM MOPS buffer | Methanosarcina thermophila | |
1300 | - |
CO2 | pH 7.5, 25°C, variant E84Q, 50 mM MOPS buffer | Methanosarcina thermophila | |
1900 | - |
CO2 | pH 7.5, 25°C, variant E84K, 50 mM MOPS buffer | Methanosarcina thermophila | |
4700 | - |
CO2 | pH 7.5, 25°C, variant E62D/E84D, 50 mM MOPS buffer | Methanosarcina thermophila | |
7100 | - |
CO2 | pH 5.7-9.5, 25°C, wild-type | Methanosarcina thermophila | |
9300 | - |
CO2 | pH 6.5, 25°C, wild-type, D2O | Methanosarcina thermophila | |
9500 | - |
CO2 | pH 7.5, 25°C, variant E84H, 50 mM MOPS buffer | Methanosarcina thermophila | |
16700 | - |
CO2 | pH 7.5, 25°C, wild-type, D2O | Methanosarcina thermophila | |
16800 | - |
CO2 | pH 7.5, 25°C, variant E62D, 50 mM MOPS buffer | Methanosarcina thermophila | |
18600 | - |
CO2 | pH 6.5, 25°C, wild-type | Methanosarcina thermophila | |
21000 | - |
CO2 | pH 7.5, 25°C, variant E84D, 50 mM MOPS buffer | Methanosarcina thermophila | |
22600 | - |
CO2 | pH 8.5, 25°C, wild-type, D2O | Methanosarcina thermophila | |
24700 | - |
CO2 | pH 7.5, 25°C, wild-type, 50 mM MOPS-Ac buffer | Methanosarcina thermophila | |
26500 | - |
CO2 | pH 7.5, 25°C, variant E88A, 50 mM MOPS buffer | Methanosarcina thermophila | |
29100 | - |
CO2 | pH 7.5, 25°C, variant E89A, 50 mM MOPS buffer | Methanosarcina thermophila | |
35100 | - |
CO2 | pH 7.5, 25°C, variant E84A, 50 mM IMID buffer | Methanosarcina thermophila | |
56800 | - |
CO2 | pH 7.5, 25°C, wild-type, 50 mM MOPS buffer | Methanosarcina thermophila | |
58900 | - |
CO2 | pH 8.5, 25°C, wild-type | Methanosarcina thermophila | |
71900 | - |
CO2 | pH 7.5, 25°C, wild-type, 50 mM IMID buffer | Methanosarcina thermophila |