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Literature summary for 4.2.1.1 extracted from

  • Tripp, B.C.; Ferry, J.G.
    A structure-function study of a proton transport pathway in teh gamma-class carbonic anhydrase from Methanosarcina thermophila (2000), Biochemistry, 39, 9232-9240.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) or Bl21(DE3) Gold Methanosarcina thermophila

Protein Variants

Protein Variants Comment Organism
E62A site-directed mutagenesis, large decrease in kcat Methanosarcina thermophila
E62A/E84A site-directed mutagenesis, large decrease in kcat Methanosarcina thermophila
E62C site-directed mutagenesis, large decrease in kcat Methanosarcina thermophila
E62D site-directed mutagenesis, 3fold decrease in kcat Methanosarcina thermophila
E62D/E84D site-directed mutagenesis, large decrease in kcat Methanosarcina thermophila
E62H site-directed mutagenesis, large decrease in kcat Methanosarcina thermophila
E62Q site-directed mutagenesis, large decrease in kcat Methanosarcina thermophila
E62T site-directed mutagenesis, large decrease in kcat Methanosarcina thermophila
E62Y site-directed mutagenesis, large decrease in kcat Methanosarcina thermophila
E84 A site-directed mutagenesis, large decrease in kcat values in pH 7.5 MOPS buffer Methanosarcina thermophila
E84 C site-directed mutagenesis, large decrease in kcat values in pH 7.5 MOPS buffer Methanosarcina thermophila
E84 D site-directed mutagenesis, large decrease in kcat values in pH 7.5 MOPS buffer Methanosarcina thermophila
E84 H site-directed mutagenesis, large decrease in kcat values in pH 7.5 MOPS buffer Methanosarcina thermophila
E84 K site-directed mutagenesis, large decrease in kcat values in pH 7.5 MOPS buffer Methanosarcina thermophila
E84 Q site-directed mutagenesis, large decrease in kcat values in pH 7.5 MOPS buffer Methanosarcina thermophila
E84 S site-directed mutagenesis, large decrease in kcat values in pH 7.5 MOPS buffer Methanosarcina thermophila
E84 Y site-directed mutagenesis, large decrease in kcat values in pH 7.5 MOPS buffer Methanosarcina thermophila
E88A site-directed mutagenesis, large decrease in kcat Methanosarcina thermophila
E89A site-directed mutagenesis, large decrease in kcat Methanosarcina thermophila

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.8
-
CO2 pH 7.5, 25°C, variant E84A, 50 mM MOPS-Ac buffer Methanosarcina thermophila
1.1
-
CO2 pH 7.5, 25°C, variant E84C, 50 mM MOPS buffer Methanosarcina thermophila
1.2
-
CO2 pH 7.5, 25°C, variant E84Q, 50 mM MOPS buffer Methanosarcina thermophila
1.4
-
CO2 pH 7.5, 25°C, variant E62Q, 50 mM MOPS buffer Methanosarcina thermophila
1.5
-
CO2 pH 7.5, 25°C, variant E84A and E84K, 50 mM MOPS buffer Methanosarcina thermophila
2.1
-
CO2 pH 7.5, 25°C, variant E84S and E84Y, 50 mM MOPS buffer Methanosarcina thermophila
2.5
-
CO2 pH 7.5, 25°C, variant E62T, 50 mM MOPS buffer Methanosarcina thermophila
2.9
-
CO2 pH 7.5, 25°C, variant E62A/E84A, 50 mM MOPS buffer Methanosarcina thermophila
3
-
CO2 pH 7.5, 25°C, variant E62Y, 50 mM MOPS buffer Methanosarcina thermophila
3.2
-
CO2 pH 7.5, 25°C, variant E84H, 50 mM MOPS buffer Methanosarcina thermophila
3.8
-
CO2 pH 7.5, 25°C, variant E62C, 50 mM MOPS buffer Methanosarcina thermophila
5.4
-
CO2 pH 7.5, 25°C, variant E62D/E84D, 50 mM MOPS buffer Methanosarcina thermophila
5.5
-
CO2 pH 7.5, 25°C, variant E62A, 50 mM MOPS-Ac buffer Methanosarcina thermophila
6.5
-
CO2 pH 7.5, 25°C, variant E62A, 50 mM MOPS buffer Methanosarcina thermophila
7.6
-
CO2 pH 7.5, 25°C, variant E62H, 50 mM MOPS buffer Methanosarcina thermophila
8.8
-
CO2 pH 7.5, 25°C, variant E84A, 50 mM IMID buffer Methanosarcina thermophila
9.4
-
CO2 pH 7.5, 25°C, variant E62A, 50 mM IMID buffer Methanosarcina thermophila
12.6
-
CO2 pH 8.5, 25°C, wild-type, D2O Methanosarcina thermophila
14.7
-
CO2 pH 7.5, 25°C, wild-type, D2O Methanosarcina thermophila
16.9
-
CO2 pH 7.5, 25°C, variant E88A, 50 mM MOPS buffer Methanosarcina thermophila
18.1
-
CO2 pH 7.5, 25°C, variant E84D, 50 mM MOPS buffer Methanosarcina thermophila
18.8
-
CO2 pH 7.5, 25°C, variant E62D, 50 mM MOPS buffer Methanosarcina thermophila
19.3
-
CO2 pH 7.5, 25°C, variant E89A, 50 mM MOPS buffer Methanosarcina thermophila
20
-
CO2 pH 8.5, 25°C, wild-type, H2O Methanosarcina thermophila
22.5
-
CO2 pH 7.5, 25°C, wild-type, 50 mM MOPS-Ac buffer Methanosarcina thermophila
23.8
-
CO2 pH 6.5, 25°C, wild-type, D2O Methanosarcina thermophila
27.8
-
CO2 pH 7.5, 25°C, wild-type, 50 mM MOPS buffer Methanosarcina thermophila
28.5
-
CO2 pH 6.5, 25°C, wild-type Methanosarcina thermophila
44.1
-
CO2 pH 7.5, 25°C, wild-type, 50 mM IMID buffer Methanosarcina thermophila

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+
-
Methanosarcina thermophila

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22873
-
3 * 22873, determined by measuring A280S of protein solution Methanosarcina thermophila
70000
-
gel filtration Methanosarcina thermophila

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
H2CO3 Methanosarcina thermophila
-
CO2 + H2O
-
r

Organism

Organism UniProt Comment Textmining
Methanosarcina thermophila
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Methanosarcina thermophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2CO3
-
Methanosarcina thermophila CO2 + H2O
-
r

Subunits

Subunits Comment Organism
trimer 3 * 22873, determined by measuring A280S of protein solution Methanosarcina thermophila

Synonyms

Synonyms Comment Organism
Cam
-
Methanosarcina thermophila
carbonic anhydrase
-
Methanosarcina thermophila
gamma-class carbonic anhydrase
-
Methanosarcina thermophila

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
47
-
CO2 pH 7.5, 25°C, variant E62Y, 50 mM MOPS buffer Methanosarcina thermophila
210
-
CO2 pH 7.5, 25°C, variant E62A/E84A, 50 mM MOPS buffer Methanosarcina thermophila
340
-
CO2 pH 7.5, 25°C, variant E62A, 50 mM MOPS-Ac buffer Methanosarcina thermophila
410
-
CO2 pH 7.5, 25°C, variant E62Q, 50 mM MOPS buffer Methanosarcina thermophila
510
-
CO2 pH 7.5, 25°C, variant E84Y, 50 mM MOPS buffer Methanosarcina thermophila
720
-
CO2 pH 7.5, 25°C, variant E84C and E62A, 50 mM MOPS buffer Methanosarcina thermophila
800
-
CO2 pH 7.5, 25°C, variant E84A, 50 mM MOPS-Ac buffer Methanosarcina thermophila
860
-
CO2 pH 7.5, 25°C, variant E62T, 50 mM MOPS buffer Methanosarcina thermophila
870
-
CO2 pH 7.5, 25°C, variant E84A, 50 mM MOPS buffer Methanosarcina thermophila
910
-
CO2 pH 7.5, 25°C, variant E62C, 50 mM MOPS buffer Methanosarcina thermophila
1050
-
CO2 pH 7.5, 25°C, variant E62H, 50 mM MOPS buffer Methanosarcina thermophila
1070
-
CO2 pH 7.5, 25°C, variant E62A, 50 mM IMID buffer Methanosarcina thermophila
1080
-
CO2 pH 7.5, 25°C, variant E84S, 50 mM MOPS buffer Methanosarcina thermophila
1300
-
CO2 pH 7.5, 25°C, variant E84Q, 50 mM MOPS buffer Methanosarcina thermophila
1900
-
CO2 pH 7.5, 25°C, variant E84K, 50 mM MOPS buffer Methanosarcina thermophila
4700
-
CO2 pH 7.5, 25°C, variant E62D/E84D, 50 mM MOPS buffer Methanosarcina thermophila
7100
-
CO2 pH 5.7-9.5, 25°C, wild-type Methanosarcina thermophila
9300
-
CO2 pH 6.5, 25°C, wild-type, D2O Methanosarcina thermophila
9500
-
CO2 pH 7.5, 25°C, variant E84H, 50 mM MOPS buffer Methanosarcina thermophila
16700
-
CO2 pH 7.5, 25°C, wild-type, D2O Methanosarcina thermophila
16800
-
CO2 pH 7.5, 25°C, variant E62D, 50 mM MOPS buffer Methanosarcina thermophila
18600
-
CO2 pH 6.5, 25°C, wild-type Methanosarcina thermophila
21000
-
CO2 pH 7.5, 25°C, variant E84D, 50 mM MOPS buffer Methanosarcina thermophila
22600
-
CO2 pH 8.5, 25°C, wild-type, D2O Methanosarcina thermophila
24700
-
CO2 pH 7.5, 25°C, wild-type, 50 mM MOPS-Ac buffer Methanosarcina thermophila
26500
-
CO2 pH 7.5, 25°C, variant E88A, 50 mM MOPS buffer Methanosarcina thermophila
29100
-
CO2 pH 7.5, 25°C, variant E89A, 50 mM MOPS buffer Methanosarcina thermophila
35100
-
CO2 pH 7.5, 25°C, variant E84A, 50 mM IMID buffer Methanosarcina thermophila
56800
-
CO2 pH 7.5, 25°C, wild-type, 50 mM MOPS buffer Methanosarcina thermophila
58900
-
CO2 pH 8.5, 25°C, wild-type Methanosarcina thermophila
71900
-
CO2 pH 7.5, 25°C, wild-type, 50 mM IMID buffer Methanosarcina thermophila