Literature summary for 4.2.1.1 extracted from

Provart, N.J.; Majeau, N.; Coleman, J.R.
Characterization of pea chloroplastic carbonic anhydrase. Expression in Escherichia coli and site-directed mutagenesis (1993), Plant Mol. Biol., 22, 937-943.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Pisum sativum

Protein Variants

Protein Variants	Comment	Organism
C160S	completely inactive	Pisum sativum
C223S	completely inactive	Pisum sativum
E204A	completely inactive	Pisum sativum
E276A	somewhat defective in catalytic activity, to varying degrees in different buffers. 40% of wild-type activity in HEPES/KOH buffer, 77.5% of wild-type activity in imidazole buffer, 6% of wild-type activity in HEPES/KOH buffer	Pisum sativum
H169N	little change in hydration activity	Pisum sativum
H209N	somewhat defective in catalytic activity, to varying degrees in different buffers, 44% of wild-type activity in imidazole buffer	Pisum sativum

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
chloroplast	-	Pisum sativum	9507	-

Organism

Organism	UniProt	Comment	Textmining
Pisum sativum	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
CO2 + H2O	-	Pisum sativum	H2CO3	-	?

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Release 2023.1 (January 2023)