Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Methanosarcina thermophila |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetazolamide | - |
Methanosarcina thermophila | |
CN- | - |
Methanosarcina thermophila | |
N3- | - |
Methanosarcina thermophila | |
OCN- | - |
Methanosarcina thermophila | |
sulfanilamide | - |
Methanosarcina thermophila |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zinc | contains 1 Zn per subunit | Methanosarcina thermophila |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22900 | - |
or tetramer, 3 * or 4 * 22900, calculation from nucleotide sequence | Methanosarcina thermophila |
22900 | - |
or trimer, 3 * or 4 * 22900, calculation from nucleotide sequence | Methanosarcina thermophila |
74000 | - |
recombinant enzyme, gel filtration | Methanosarcina thermophila |
84000 | - |
authentic enzyme, gel filtration | Methanosarcina thermophila |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina thermophila | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the discrepancy in molecular weight between the recombinant and the authentic enzyme suggests that the carbonic anhydrase is posttranslationally modified in either E. coli or Methanobacterium thermophilum | Methanosarcina thermophila |
Purification (Comment) | Organism |
---|---|
- |
Methanosarcina thermophila |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Methanosarcina thermophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CO2 + H2O | - |
Methanosarcina thermophila | H2CO3 | - |
? | |
additional information | no esterase activity | Methanosarcina thermophila | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | or trimer, 3 * or 4 * 22900, calculation from nucleotide sequence | Methanosarcina thermophila |
trimer | or tetramer, 3 * or 4 * 22900, calculation from nucleotide sequence | Methanosarcina thermophila |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
15 min, stable | Methanosarcina thermophila |