Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.1.1 extracted from

  • Alber, B.E.; Ferry, J.G.
    Characterization of heterologously produced carbonic anhydrase from Methanosarcina thermophila (1996), J. Bacteriol., 178, 3270-3274.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Methanosarcina thermophila

Inhibitors

Inhibitors Comment Organism Structure
acetazolamide
-
Methanosarcina thermophila
CN-
-
Methanosarcina thermophila
N3-
-
Methanosarcina thermophila
OCN-
-
Methanosarcina thermophila
sulfanilamide
-
Methanosarcina thermophila

Metals/Ions

Metals/Ions Comment Organism Structure
Zinc contains 1 Zn per subunit Methanosarcina thermophila

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22900
-
or tetramer, 3 * or 4 * 22900, calculation from nucleotide sequence Methanosarcina thermophila
22900
-
or trimer, 3 * or 4 * 22900, calculation from nucleotide sequence Methanosarcina thermophila
74000
-
recombinant enzyme, gel filtration Methanosarcina thermophila
84000
-
authentic enzyme, gel filtration Methanosarcina thermophila

Organism

Organism UniProt Comment Textmining
Methanosarcina thermophila
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the discrepancy in molecular weight between the recombinant and the authentic enzyme suggests that the carbonic anhydrase is posttranslationally modified in either E. coli or Methanobacterium thermophilum Methanosarcina thermophila

Purification (Commentary)

Purification (Comment) Organism
-
Methanosarcina thermophila

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Methanosarcina thermophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CO2 + H2O
-
Methanosarcina thermophila H2CO3
-
?
additional information no esterase activity Methanosarcina thermophila ?
-
?

Subunits

Subunits Comment Organism
tetramer or trimer, 3 * or 4 * 22900, calculation from nucleotide sequence Methanosarcina thermophila
trimer or tetramer, 3 * or 4 * 22900, calculation from nucleotide sequence Methanosarcina thermophila

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
15 min, stable Methanosarcina thermophila