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Literature summary for 4.1.99.5 extracted from
- Solubilization, partial purification, and characterization of a fatty aldehyde decarbonylase from a higher plant, Pisum sativum (2000), Arch. Biochem. Biophys., 377, 341-349.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| phopshatidylserine | requirement, phosphatidylcholine is preferred, phopshatidylserine and phopshatidylethanolamine substitute less effectively | Pisum sativum |  |
| phosphatidylcholine | requirement, preferred lipid, phopshatidylserine and phopshatidylethanolamine substitute less effectively | Pisum sativum | |
| phosphatidylethanolamine | requirement, phosphatidylcholine is preferred, phopshatidylserine and phopshatidylethanolamine substitute less effectively | Pisum sativum | |
| Phospholipid | requirement, dose-dependent stimulation, phosphatidylcholine is preferred, phopshatidylserine and phopshatidylethanolamine substitute less effectively | Pisum sativum | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | 1 mM, complete inhibition, CuCl2 restores, CoCl2 and ZnCl2 partially restore activity; strong | Pisum sativum | |
| dithioerythritol | 5 mM, complete inhibition | Pisum sativum | |
| EDTA | 10 mM, 58% inhibition | Pisum sativum | |
| metal ion chelators | Cu2+ fully restores activity; strong | Pisum sativum | |
| NADH | presence causes 50% inhibition | Pisum sativum | |
| NADPH | presence causes 50% inhibition | Pisum sativum | |
| O2 | complete inhibition | Pisum sativum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | membrane-associated | Pisum sativum | 16020 | - |
| microsome | membrane-associated | Pisum sativum | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | probably a copper enzyme | Pisum sativum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 67000 | - |
x * 67000, SDS-PAGE | Pisum sativum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Pisum sativum | alkane biosynthesis | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pisum sativum | - |
L. V Early Frosty | - |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| inhibited in the presence of O2 | Pisum sativum |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, 1306fold, solubilized with beta-octyl glucoside | Pisum sativum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | young rapidly growing | Pisum sativum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.000235 | - |
at room temperature | Pisum sativum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | alkane biosynthesis | Pisum sativum | ? | - |
? | |
| octadecanal | - |
Pisum sativum | heptadecane + CO | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 67000, SDS-PAGE | Pisum sativum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
assay at room temperature | Pisum sativum |
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