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Literature summary for 4.1.99.3 extracted from

  • Cahoon, L.A.; Stohl, E.A.; Seifert, H.S.
    The Neisseria gonorrhoeae photolyase orthologue phrB is required for proper DNA supercoiling but does not function in photo-reactivation (2011), Mol. Microbiol., 79, 729-742.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.99.3

Cloned(Commentary)

Cloned (Comment) Organism
gene phrB, recombinant expression in Escherichia coli enzyme-deficient mutant strain CSR603. The Neisseria gonorrhoeae phrB cannot complement an Escherichia coli phrB mutant strain CSR603 Neisseria gonorrhoeae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Neisseria gonorrhoeae PhrB does not function as a photolyase ?
-
 ?
additional information Neisseria gonorrhoeae FA 1090 PhrB does not function as a photolyase ?
-
 ?

Organism

Organism UniProt Comment Textmining
Neisseria gonorrhoeae Q5F659 gene phrB or NGO1707
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Neisseria gonorrhoeae FA 1090 Q5F659 gene phrB or NGO1707
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information PhrB does not function as a photolyase Neisseria gonorrhoeae ?
-
 ?
additional information PhrB does not function as a photolyase Neisseria gonorrhoeae FA 1090 ?
-
 ?

Synonyms

Synonyms Comment Organism
photolyase orthologue
-
 Neisseria gonorrhoeae
PhrB
-
 Neisseria gonorrhoeae
PhrB orthologue
-
 Neisseria gonorrhoeae

General Information

General Information Comment Organism
evolution the PhrB sequence is conserved in both pathogenic and commensal Neisseria species but shares little identity to other bacterial species, phylogenetic analysis, overview. The gonococcal PhrB gene is not a functional orthologue of the Escherichia coli PhrB Neisseria gonorrhoeae
malfunction loss of phrB does not result in a mutator phenotype. A Neisseria gonorrhoeae phrB mutant has a reduced colony size that is not a result of a growth defect and the mutant cells exhibit an altered morphology. Although the phrB mutant exhibits increased sensitivity to oxidative killing, it shows increased survival on media containing nalidixic acid or rifampicin, but does not have an increased mutation rate with these antibiotics or spectinomycin and kasugamycin. The phrB mutant shows increased negative DNA supercoiling, but while the protein bound double-stranded DNA, it does not express topoisomerase activity. The Neisseria gonorrhoeae phrB cannot complement an Escherichia coli phrB mutant strain CSR603, and the Neisseria gonorrhoeae phrB mutant is not more sensitive to UV irradiation, independent of visible light exposure, phenotype, overview Neisseria gonorrhoeae
physiological function the enzyme PhrB has a role in maintaining DNA supercoiling that is important for normal cell physiology Neisseria gonorrhoeae