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Literature summary for 4.1.99.3 extracted from
- Detection of distinct alpha-helical rearrangements of cyclobutane pyrimidine dimer photolyase upon substrate binding by Fourier transform infrared spectroscopy (2013), Biochemistry, 52, 1019-1027.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 5,10-methenyltetrahydrofolate | binds to the enzyme and acts as a light-harvesting pigment | Escherichia coli |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E109A | site-directed mutagenesis, inactive mutant | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cyclobutadipyrimidine (in DNA) | Escherichia coli | photolyases utilize near-ultraviolet blue light to specifically repair the major photoproducts of UV-induced damaged DNA. The enzyme specifically repairs CPD lesions | 2 pyrimidine residues (in DNA) | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by cobalt affinity chromatography | Escherichia coli |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| cyclobutadipyrimidine (in DNA) = 2 pyrimidine residues (in DNA) | reaction mechanism, overview | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cyclobutadipyrimidine (in DNA) | photolyases utilize near-ultraviolet blue light to specifically repair the major photoproducts of UV-induced damaged DNA. The enzyme specifically repairs CPD lesions | Escherichia coli | 2 pyrimidine residues (in DNA) | - |
? | |
| cyclobutadipyrimidine (in DNA) | repair of a single CPD lesion within a double-stranded DNA molecule | Escherichia coli | 2 pyrimidine residues (in DNA) | - |
? | |
| additional information | the enzyme shows light-induced reduction of FAD, and photorepair involves the transfer of an electron from the photoexcited reduced FAD to the damaged DNA for cleaving the dimers to maintain the DNA's integrity, substrate specificity, overview | Escherichia coli | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CPD-Phr | - |
Escherichia coli |
| cyclobutane pyrimidine dimer photolyase | - |
Escherichia coli |
| cyclobutane pyrimidine dimer PHR | - |
Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | light-induced reduction of FAD, and transfer of an electron from the photoexcited reduced FAD to the damaged DNA for cleaving the dimers | Escherichia coli | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | repair mechanism and the substrate specificity that distinguish enzyme CPD-PHR from enzyme (6-4) PHR, EC 4.1.99.13, which uniquely repairs (6-4) photoproducts, using Fourier transform infrared spectroscopy, overview | Escherichia coli |
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