Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli, the purified native rice CPD photolyase has significantly higher CPD photorepair activity than the Escherichia coli-expressed CPD photolyase, the structure of the native rice CPD photolyase differs significantly from that of the Escherichia coli-expressed rice CPD photolyase, and the structural modification of the native CPD photolyase leads to higher activity | Oryza sativa Japonica Group |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
purified native unphosphorylated CPD photolyase bound to CPD-containing DNA conjugated to magnetic beads and then released by blue-light irradiation, SDS-PAGE | Oryza sativa Japonica Group |
55000 | - |
unphosphorylated recombinant enzyme, SDS-PAGE | Oryza sativa Japonica Group |
56000 | - |
purified native phosphorylated CPD photolyase bound to CPD-containing DNA conjugated to magnetic beads and then released by blue-light irradiation, SDS-PAGE | Oryza sativa Japonica Group |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Oryza sativa Japonica Group | the native rice CPD photolyase is phosphorylated, whereas the Escherichia coli-expressed rice CPD photolyase is not | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa Japonica Group | Q6F6A2 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | the native rice CPD photolyase is phosphorylated, whereas the Escherichia coli-expressed rice CPD photolyase is not | Oryza sativa Japonica Group |
Purification (Comment) | Organism |
---|---|
ammonium sulfate fractionation, UNO-Q12 column chromatography, and heparin affinity column chromatography, the purified native rice CPD photolyase has significantly higher CPD photorepair activity than the Escherichia coli-expressed CPD photolyase | Oryza sativa Japonica Group |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Oryza sativa Japonica Group | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyclobutadipyrimidine in DNA | - |
Oryza sativa Japonica Group | pyrimidine residues in DNA | - |
? | |
additional information | the native rice CPD photolyase is phosphorylated, whereas the Escherichia coli-expressed rice CPD photolyase is not | Oryza sativa Japonica Group | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 56000, SDS-PAGE | Oryza sativa Japonica Group |
Synonyms | Comment | Organism |
---|---|---|
CPD photolyase | absorbs blue/UVA light as an energy source to monomerize dimers | Oryza sativa Japonica Group |
cyclobutane pyrimidine dimer photolyase | - |
Oryza sativa Japonica Group |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Oryza sativa Japonica Group |