Application | Comment | Organism |
---|---|---|
medicine | exploring therapeutic possibilities of ectopic CPD photolyase expression | Potorous tridactylus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mutation of Q403 and K406 affect the affinity toward cyclobutane-pyrimidine dimer-containing DNA | Escherichia coli |
additional information | mutation of Q411 and K414 affect the affinity toward cyclobutane-pyrimidine dimer-containing DNA | Aspergillus nidulans |
additional information | transgenic mice are generated with a transgene for a marsupial CPD photolyase. These mice exhibit a 40% increase of repair for CPD lesions in intact skin and cultured fibroblasts that is accompanied by an improved resistance against acute UV-induced effects like erythema (sunburn), epidermal hyperplasia or apoptosis. Expression of the CPD photolyase in mice efficiently suppresses the formation of skin carcinomas | Potorous tridactylus |
R342A | conserved arginine forms a salt bridge with phosphate+1. Among several active-site mutants this R/A mutant is found to exhibit the greatest effect by lowering the selectivity toward cyclobutane-pyrimidine dimer-containing DNA 32-fold and the quantum yield of CPD cleavage from 98% to about 60% | Escherichia coli |
R350A | conserved arginine forms a salt bridge with phosphate+1. Among several active-site mutants this R/A mutant is found to exhibit the greatest effect by lowering the selectivity toward cyclobutane-pyrimidine dimer-containing DNA 32-fold and the quantum yield of CPD cleavage from 98% to about 60% | Aspergillus nidulans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Aspergillus nidulans | - |
- |
- |
Escherichia coli | P00914 | - |
- |
Potorous tridactylus | - |
- |
- |
Synonyms | Comment | Organism |
---|---|---|
CPD photolyase | - |
Potorous tridactylus |
Cry1 | - |
Aspergillus nidulans |
Cry1 | - |
Arabidopsis thaliana |
DNA photolyase | - |
Aspergillus nidulans |
DNA photolyase | - |
Arabidopsis thaliana |
DNA photolyase | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FADH2 | the isoalloxazine ring is sandwiched between a salt bridge comprising an arginine and an aspartate residue | Arabidopsis thaliana | |
FADH2 | the isoalloxazine ring is sandwiched between a salt bridge comprising an arginine and an aspartate residue: R344/D372 and short polypeptide stretches: A377-N378/G381-W382. Another conserved interaction is a hydrogen bond formed between the N5 nitrogen of the isoalloxazine group and a conserved asparagine: N378 | Escherichia coli | |
FADH2 | the isoalloxazine ring is sandwiched between a salt bridge comprising an arginine and an aspartate residue: R352/D380 and short polypeptide stretches: A385-N386/G389-W390. Another conserved interaction is a hydrogen bond formed between the N5 nitrogen of the isoalloxazine group and a conserved asparagine: N386. Mutagenesis and ultrafast kinetic spectroscopy revealed a consecutive chain of three conserved tryptophan residues with the order: Y469 -(?)- W314- W367- W390- FAD(H) | Aspergillus nidulans |