Crystallization (Comment) | Organism |
---|---|
tyrosine phenol-lyase complexed with 3-(4'-hydroxyphenyl)propionic acid | Citrobacter freundii |
Protein Variants | Comment | Organism |
---|---|---|
R381A | beta-elimination activity has been reduced by 0.0001fold compared to wild type enzyme | Citrobacter freundii |
R381I | no detectable beta-elimination activity with L-Tyr as substrate | Citrobacter freundii |
R381V | no detectable beta-elimination activity with L-Tyr as substrate | Citrobacter freundii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrobacter freundii | P31013 | wild type enzyme and mutant enzymes R381A, R381V and R381I | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
O-benzoyl-L-Ser + H2O | - |
Citrobacter freundii | ? | - |
? | |
O-benzyl-L-Cys + H2O | - |
Citrobacter freundii | ? | - |
? | |
O-benzyl-L-Ser + H2O | - |
Citrobacter freundii | ? | - |
? | |
S-benzyl-L-Cys + H2O | - |
Citrobacter freundii | ? + pyruvate + NH3 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the turnover-numbers of the mutant enzyme R381I, R381V and R381A for the substrates S-(o-nitrophenyl)-L-Cys, 3-chloro-L-Ala, O-benzoyl-L-Ser and S-methyl-L-Cys are comparable to those of the wild-type enzyme | Citrobacter freundii | |
0.5 | - |
S-benzyl-L-Cys | wild-type enzyme | Citrobacter freundii | |
0.9 | - |
S-methyl-L-Cys | wild-type enzyme | Citrobacter freundii | |
1.7 | - |
S-ethyl-L-Cys | wild-type enzyme | Citrobacter freundii | |
2.2 | - |
L-Tyr | wild-type enzyme | Citrobacter freundii | |
3 | - |
3-chloro-L-Ala | wild-type enzyme | Citrobacter freundii | |
3.9 | - |
O-benzyl-L-Cys | wild-type enzyme | Citrobacter freundii | |
5.1 | - |
S-(o-nitrophenyl)-L-Cys | wild-type enzyme | Citrobacter freundii | |
8.3 | - |
O-benzoyl-L-Ser | wild-type enzyme | Citrobacter freundii | |
9.7 | - |
S-(o-nitrophenyl)-L-Cys | wild-type enzyme | Citrobacter freundii |