Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xenopus laevis | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(6-4) photoproduct (in DNA) = 2 pyrimidine residues (in DNA) | Fourier transform infrared spectroscopy is applied to (6-4)DNA photolyase. Differences in FTIR spectra that correspond to (6-4)DNA photolyase photoactivation, substrate binding, and light-dependent DNA repair processes are reported. The presence of DNA carrying a single (6-4) PP uniquely influences vibrations of the protein backbone and a protonated carboxylic acid, whereas photoactivation produces IR spectral changes for the FAD cofactor and the surrounding protein. Difference FTIR spectra for the light-dependent DNA damage repair reaction directly show significant DNA structural changes in the (6-4) lesion and the neighboring phosphate group. Time-dependent illumination of samples with different enzyme:substrate stoichiometries successfully distinguish signals characteristic of structural changes in the protein and the DNA resulting from binding and catalysis | Xenopus laevis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(6-4) photoproduct (in DNA) | - |
Xenopus laevis | 2 pyrimidine residues (in DNA) | - |
? |
Synonyms | Comment | Organism |
---|---|---|
(6-4) PHR | - |
Xenopus laevis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Xenopus laevis |