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Literature summary for 4.1.99.13 extracted from

  • Chen, J.J.; Mitchell, D.L.; Britt, A.B.
    A light-dependent pathway for the elimination of UV-induced pyrimidine (6-4) pyrimidinone photoproducts in Arabidopsis (1994), Plant Cell, 6, 1311-1317.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.99.13

Protein Variants

Protein Variants Comment Organism
additional information light-dependent repair of UV-induced (6-4) photoproducts is investigated in an excision repair-deficient Arabidopsis mutant. It is demonstrated that (6-4) photoproducts are efficiently eliminated in a light-dependent manner which occurs in the presence of blue light (435 nm) but not upon exposure to light of longer wavelengths Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Reaction

Reaction Comment Organism Reaction ID
(6-4) photoproduct (in DNA) = 2 pyrimidine residues (in DNA) The overall repair reaction consists of two distinct steps, one of which is light-independent and the other one light-dependent. In the initial light-independent step, a 6-iminium ion is thought to be generated via proton transfer induced by two histidines highly conserved among the (6-4) photolyases.This intermediate spontaneously rearranges to form an oxetane intermediate by intramolecular nucleophilic attack. In the subsequent light-driven reaction, one electron is believed to be transferred from the fully reduced FAD cofactor (FADH-) to the oxetane intermediate thus forming a neutral FADH radical and an anionic oxetane radical, which spontaneously fractures. The excess electron is then back-transferred to the flavin radical restoring the fully reduced flavin cofactor and a pair of pyrimidine bases Arabidopsis thaliana
a

Source Tissue

Source Tissue Comment Organism Textmining
additional information it is found that the 6-4 photoreactivating activity is constitutively expressed prior to as well as during the period of repair Arabidopsis thaliana
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seedling
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(6-4) photoproduct (in DNA)
-
 Arabidopsis thaliana 2 pyrimidine residues (in DNA)
-
 ?

Synonyms

Synonyms Comment Organism
(6-4) photolyase
-
 Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Arabidopsis thaliana