Literature summary for 4.1.99.13 extracted from

Todo, T.; Tsuji, H.; Otoshi, E.; Hitomi, K.; Kim, S.T.; Ikenaga, M.
Characterization of a human homolog of (6-4) photolyase (1997), Mutat. Res., 384, 195-204.

Search for more literature for 4.1.99.13

Cloned(Commentary)

Cloned (Comment)	Organism
expressed as a GSTtagged fusion protein in Escherichia coli	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	expression of H64PRH does not show any photoreactivating effects on the survival of UV-irradiated Escherichia coli. Using a gel shift assay with with un-irradiated and UV-irradiated DNA probes it is shown that H64PRH protein does not possess any binding activity to either DNA probe	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
63000	-	SDS-PAGE	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
by using a glutathione sepharose column and a Hi Trap Q column. Concentrated fusion protein is cleaved with thrombin from bovine plasma by incubation overnight at 4°C. For chromophore determination, the eluate from the glutathione sepharose column is purified through a Q sepharose column, omitting gel filtration procedure, and concentrated by ultrafiltration	Homo sapiens

Reaction

Reaction	Comment	Organism	Reaction ID
(6-4) photoproduct (in DNA) = 2 pyrimidine residues (in DNA)	The overall repair reaction consists of two distinct steps, one of which is light-independent and the other one light-dependent. In the initial light-independent step, a 6-iminium ion is thought to be generated via proton transfer induced by two histidines highly conserved among the (6-4) photolyases.This intermediate spontaneously rearranges to form an oxetane intermediate by intramolecular nucleophilic attack. In the subsequent light-driven reaction, one electron is believed to be transferred from the fully reduced FAD cofactor (FADH-) to the oxetane intermediate thus forming a neutral FADH radical and an anionic oxetane radical, which spontaneously fractures. The excess electron is then back-transferred to the flavin radical restoring the fully reduced flavin cofactor and a pair of pyrimidine bases	Homo sapiens	a

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	by Northern blot analysis an ubiquitous expression pattern is demonstrated	Homo sapiens	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	enzyme does not show any detectable photolyase activity	Homo sapiens

Synonyms

Synonyms	Comment	Organism
(6-4) photolyase	-	Homo sapiens
H64PRH	-	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Homo sapiens

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Release 2023.1 (January 2023)