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Literature summary for 4.1.99.13 extracted from

  • Yan Lv, X.; Rong Qiao, D.; Xiong, Y.; Xu, H.; You, F.F.; Cao, Y.; He, X.
    Photoreactivation of (6-4) photolyase in Dunaliella salina (2008), FEMS Microbiol. Lett., 283, 42-46.
    View publication on PubMed
Search for more literature for 4.1.99.13

Cloned(Commentary)

Cloned (Comment) Organism
expressed as a GST-tagged fusion protein in Escherichia coli Dunaliella salina

Metals/Ions

Metals/Ions Comment Organism Structure
additional information enzyme is also active under high salinity Dunaliella salina
additional information NH4Cl or NaH2PO4 increase activity of the enzyme. CH3COONa or Na2CO3 strongly decrease the enzyme’s activity. Maximum activity occurs in the presence of NaH2PO4, which is increased eight times than that in the presence of Na2CO3. When the ions that possess stronger ability to donate a proton are added to enzyme reaction buffer, the rate of photoreactivation increases Dunaliella salina
Na+
-
 Dunaliella salina

Organism

Organism UniProt Comment Textmining
Dunaliella salina
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by using glutathione-sepharose columns Dunaliella salina

Reaction

Reaction Comment Organism Reaction ID
(6-4) photoproduct (in DNA) = 2 pyrimidine residues (in DNA) The overall repair reaction consists of two distinct steps, one of which is light-independent and the other one light-dependent. In the initial light-independent step, a 6-iminium ion is thought to be generated via proton transfer induced by two histidines highly conserved among the (6-4) photolyases.This intermediate spontaneously rearranges to form an oxetane intermediate by intramolecular nucleophilic attack. In the subsequent light-driven reaction, one electron is believed to be transferred from the fully reduced FAD cofactor (FADH-) to the oxetane intermediate thus forming a neutral FADH radical and an anionic oxetane radical, which spontaneously fractures. The excess electron is then back-transferred to the flavin radical restoring the fully reduced flavin cofactor and a pair of pyrimidine bases Dunaliella salina
a

Source Tissue

Source Tissue Comment Organism Textmining
additional information expression levels of (6–4) photolyase in 24 h dark-maintained algae increase rapidly after 8-h exposure to white light, and also increase after being treated with NaCl for 8 h Dunaliella salina
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 enzyme shows activity with the GST tag Dunaliella salina

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(6-4) photoproduct (in DNA)
-
 Dunaliella salina pyrimidine residues (in DNA)
-
 ?

Synonyms

Synonyms Comment Organism
(6-4) photolyase
-
 Dunaliella salina

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 maximum activity occurs at pH 8.5 and is 2.4fold higher than that at pH 7.0 Dunaliella salina

pH Range

pH Minimum pH Maximum Comment Organism
8.5 9 highest activity at Dunaliella salina

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Dunaliella salina