Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21DE3 cells, the vectors pET-28b, pACYC-Duet, pET-Duet and pRSF-Duet are used | Thauera aromatica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
7000 | - |
gamma subunit, determined by SDS-PAGE | Thauera aromatica |
14000 | - |
beta subunit, determined by SDS-PAGE | Thauera aromatica |
100000 | - |
alpha subunit, determined by SDS-PAGE | Thauera aromatica |
230000 | - |
determined by gel filtration, consistent with an alpha2beta2gamma2 subunit structure | Thauera aromatica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
toluene + fumarate | Thauera aromatica | - |
benzylsuccinate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thauera aromatica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using a Ni-NTA-agarose column | Thauera aromatica |
Renatured (Comment) | Organism |
---|---|
the alpha subunit is purified and refolded from inclusion bodies | Thauera aromatica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
toluene + fumarate | - |
Thauera aromatica | benzylsuccinate | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotrimer | alpha, beta and gamma subunit | Thauera aromatica |
hexamer | the structural model for benzylsuccinate synthase proposes an alpha2beta2gamma2-complex | Thauera aromatica |
Synonyms | Comment | Organism |
---|---|---|
benzylsuccinate synthase | - |
Thauera aromatica |
BSS | - |
Thauera aromatica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
iron-sulfur centre | - |
Thauera aromatica |