Cloned (Comment) | Organism |
---|---|
expressed as glutathione S-transferase fusion protein | Prevotella intermedia |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.23 | - |
L-tryptophan | pH 7.5, 37°C | Prevotella intermedia | |
0.87 | - |
S-methyl-L-cysteine | pH 7.6, 37°C | Prevotella intermedia | |
3.3 | - |
S-ethyl-L-cysteine | pH 7.6, 37°C | Prevotella intermedia |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
2 * 51000, SDS-PAGE, gel filtration | Prevotella intermedia |
107800 | - |
gel filtration | Prevotella intermedia |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Prevotella intermedia | E9RK37 | - |
- |
Prevotella intermedia ATCC 25611 | E9RK37 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Prevotella intermedia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tryptophan + H2O | - |
Prevotella intermedia | indole + pyruvate + NH3 | - |
? | |
L-tryptophan + H2O | - |
Prevotella intermedia ATCC 25611 | indole + pyruvate + NH3 | - |
? | |
S-ethyl-L-cysteine + H2O | - |
Prevotella intermedia | ethanethiol + pyruvate + NH4+ | - |
? | |
S-ethyl-L-cysteine + H2O | - |
Prevotella intermedia ATCC 25611 | ethanethiol + pyruvate + NH4+ | - |
? | |
S-methyl-L-cysteine + H2O | - |
Prevotella intermedia | methanethiol + pyruvate + NH4+ | - |
? | |
S-methyl-L-cysteine + H2O | - |
Prevotella intermedia ATCC 25611 | methanethiol + pyruvate + NH4+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 51000, SDS-PAGE, gel filtration | Prevotella intermedia |
Synonyms | Comment | Organism |
---|---|---|
TnaA | - |
Prevotella intermedia |
tryptophanase | - |
Prevotella intermedia |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
S-methyl-L-cysteine | pH 7.6, 37°C | Prevotella intermedia | |
0.45 | - |
L-tryptophan | pH 7.5, 37°C | Prevotella intermedia | |
1.79 | - |
S-ethyl-L-cysteine | pH 7.6, 37°C | Prevotella intermedia |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.23 | - |
S-methyl-L-cysteine | pH 7.6, 37°C | Prevotella intermedia | |
0.6 | - |
S-ethyl-L-cysteine | pH 7.6, 37°C | Prevotella intermedia | |
1.96 | - |
L-tryptophan | pH 77.5, 37°C | Prevotella intermedia |