Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.99.1 extracted from

  • Suzuki, S.; Hirahara, T.; Horinouchi, S.; Beppu, T.
    Purification and properties of thermostable tryptophanase from an obligately symbiotic thermophile, Symbiobacterium thermophilum (1991), Agric. Biol. Chem., 55, 3059-3066.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.47
-
L-Trp
-
Symbiobacterium thermophilum

Metals/Ions

Metals/Ions Comment Organism Structure
K+ activates Symbiobacterium thermophilum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
4 * 46000, SDS-PAGE Symbiobacterium thermophilum
210000
-
gel filtration Symbiobacterium thermophilum

Organism

Organism UniProt Comment Textmining
Symbiobacterium thermophilum
-
-
-
Symbiobacterium thermophilum T
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Symbiobacterium thermophilum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.6
-
-
Symbiobacterium thermophilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-hydroxy-L-Trp + H2O 6.7% of the activity with L-Trp Symbiobacterium thermophilum ?
-
?
5-hydroxy-L-Trp + H2O 6.7% of the activity with L-Trp Symbiobacterium thermophilum T ?
-
?
5-methyl-L-Trp + H2O 15.2% of the activity with L-Trp Symbiobacterium thermophilum ?
-
?
5-methyl-L-Trp + H2O 15.2% of the activity with L-Trp Symbiobacterium thermophilum T ?
-
?
Cys + H2O 18.9% of the activity with L-Trp Symbiobacterium thermophilum ?
-
?
Cys + H2O L-Cys Symbiobacterium thermophilum ?
-
?
Cys + H2O 18.9% of the activity with L-Trp Symbiobacterium thermophilum T ?
-
?
Cys + H2O L-Cys Symbiobacterium thermophilum T ?
-
?
indole + pyruvate + NH4+
-
Symbiobacterium thermophilum L-Trp + H2O
-
?
L-Trp + H2O
-
Symbiobacterium thermophilum indole + pyruvate + NH4+
-
?
S-methyl-L-Cys + H2O 63.8% of the activity with L-Trp Symbiobacterium thermophilum ?
-
?
S-methyl-L-Cys + H2O 63.8% of the activity with L-Trp Symbiobacterium thermophilum T ?
-
?
Ser + H2O L-Ser Symbiobacterium thermophilum ?
-
?
Ser + H2O 5.3% of the activity with L-Trp Symbiobacterium thermophilum ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 46000, SDS-PAGE Symbiobacterium thermophilum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Symbiobacterium thermophilum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
20 min stable Symbiobacterium thermophilum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
42
-
L-Trp
-
Symbiobacterium thermophilum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Symbiobacterium thermophilum

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 10 25°C, 28 h, stable Symbiobacterium thermophilum