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Literature summary for 4.1.99.1 extracted from

  • Snell, E.E.
    Tryptophanase: structure, catalytic activities, and mechanism of action (1975), Adv. Enzymol. Relat. Areas Mol. Biol., 42, 287-333.
    View publication on PubMed
Search for more literature for 4.1.99.1

Inhibitors

Inhibitors Comment Organism Structure
4-phenyl-2-aminobutyrate
-
 Escherichia coli
beta-phenyllactate
-
 Escherichia coli
beta-phenylserine
-
 Escherichia coli
Ethionine
-
 Escherichia coli
indoleacrylate
-
 Escherichia coli
indolepropionate
-
 Escherichia coli
Indolepyruvate
-
 Escherichia coli
Met
-
 Escherichia coli
Phe
-
 Aeromonas hydrophila
Phe
-
 Escherichia coli
Phe
-
 Providencia rettgeri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.019
-
 indole
-
 Escherichia coli
0.065
-
 S-benzylcysteine strain B/1t7-A, 25°C Escherichia coli
0.11
-
 O-benzylserine strain B/1t7-A Escherichia coli
0.22
-
 L-Trp
-
 Aeromonas hydrophila
0.26
-
 L-Trp
-
 Providencia rettgeri
0.27
-
 L-Trp
-
 Paenibacillus alvei
0.3
-
 L-Trp strain B/1t7-A, 25°C Escherichia coli
0.33
-
 L-Trp strain B/1t7-A, 37°C Escherichia coli
0.6
-
 alpha,beta-diaminopropionic acid strain B/1t7-A Escherichia coli
1
-
 S-ethylcysteine strain B/1t7-A, 25°C Escherichia coli
1.2
-
 beta-chloroalanine strain B/1t7-A Escherichia coli
4
-
 O-methylserine strain B/1t7-A Escherichia coli
10
-
 S-methylcysteine strain B/1t7-A, 37°C Escherichia coli
11
-
 Cys
-
 Escherichia coli
70
-
 pyruvate
-
 Escherichia coli
110
-
 cysteine sulfinic acid strain B/1t7-A Escherichia coli
345
-
 NH4+
-
 Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
208000
-
-
 Paenibacillus alvei
220000
-
 strain B/1t7-A Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Vibrio sp.
-
 ?
-
 ?
additional information Providencia rettgeri
-
 ?
-
 ?
additional information Escherichia coli induced by Trp ?
-
 ?

Organism

Organism UniProt Comment Textmining
Aeromonas hydrophila
-
-
-
Aeromonas sp.
-
-
-
Bacillus sp. (in: Bacteria)
-
-
-
Bacteroides sp.
-
-
-
Corynebacterium sp.
-
-
-
Enterobacter sp.
-
-
-
Erwinia sp.
-
-
-
Escherichia coli
-
-
-
Kluyvera sp.
-
-
-
Micrococcus sp.
-
-
-
no activity in Acetobacter sp.
-
-
-
no activity in Achromobacter sp.
-
-
-
no activity in Agrobacterium sp.
-
-
-
no activity in Alcaligenes sp.
-
-
-
no activity in Azotobacter sp.
-
-
-
no activity in Clostridium sp.
-
-
-
no activity in Flavobacterium sp.
-
-
-
no activity in Mycoplasma sp.
-
-
-
no activity in Pseudomonas sp.
-
-
-
no activity in Rhizobium sp.
-
-
-
no activity in Salmonella sp.
-
-
-
no activity in Serratia sp.
-
-
-
no activity in Xanthomonas sp.
-
-
-
Paenibacillus alvei
-
-
-
Paracolobactrum sp.
-
-
-
Pasteurella sp.
-
-
-
Photobacterium sp.
-
-
-
Proteus sp.
-
-
-
Providencia rettgeri
-
-
-
Sphaerophorus sp.
-
-
-
Vibrio sp.
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha,beta-diaminopropionic acid
-
 Escherichia coli ?
-
 ?
beta-chloroalanine + H2O
-
 Escherichia coli ?
-
 ?
Cys + H2O
-
 Escherichia coli ?
-
 ?
Cys + H2O
-
 Providencia rettgeri ?
-
 ?
Cys + indole
-
 Escherichia coli Trp + ?
-
 ?
cysteine sulfinic acid + H2O
-
 Escherichia coli ?
-
 ?
L-Trp + H2O
-
 Escherichia coli indole + pyruvate + NH4+
-
 ?
L-Trp + H2O
-
 Corynebacterium sp. indole + pyruvate + NH4+
-
 ?
L-Trp + H2O
-
 Bacillus sp. (in: Bacteria) indole + pyruvate + NH4+
-
 ?
L-Trp + H2O
-
 Aeromonas hydrophila indole + pyruvate + NH4+
-
 ?
L-Trp + H2O
-
 Vibrio sp. indole + pyruvate + NH4+
-
 ?
L-Trp + H2O
-
 Micrococcus sp. indole + pyruvate + NH4+
-
 ?
L-Trp + H2O
-
 Enterobacter sp. indole + pyruvate + NH4+
-
 ?
L-Trp + H2O
-
 Aeromonas sp. indole + pyruvate + NH4+
-
 ?
L-Trp + H2O
-
 Erwinia sp. indole + pyruvate + NH4+
-
 ?
L-Trp + H2O
-
 Proteus sp. indole + pyruvate + NH4+
-
 ?
L-Trp + H2O
-
 Bacteroides sp. indole + pyruvate + NH4+
-
 ?
L-Trp + H2O
-
 Pasteurella sp. indole + pyruvate + NH4+
-
 ?
L-Trp + H2O
-
 Providencia rettgeri indole + pyruvate + NH4+
-
 ?
L-Trp + H2O
-
 Paracolobactrum sp. indole + pyruvate + NH4+
-
 ?
L-Trp + H2O
-
 Kluyvera sp. indole + pyruvate + NH4+
-
 ?
L-Trp + H2O
-
 Photobacterium sp. indole + pyruvate + NH4+
-
 ?
L-Trp + H2O
-
 Sphaerophorus sp. indole + pyruvate + NH4+
-
 ?
L-Trp + H2O
-
 Paenibacillus alvei indole + pyruvate + NH4+
-
 ?
additional information
-
 Vibrio sp. ?
-
 ?
additional information
-
 Providencia rettgeri ?
-
 ?
additional information induced by Trp Escherichia coli ?
-
 ?
O-benzylserine + H2O
-
 Escherichia coli ?
-
 ?
O-methylserine + H2O
-
 Escherichia coli ?
-
 ?
S-benzylcysteine + H2O
-
 Escherichia coli ?
-
 ?
S-ethylcysteine + H2O
-
 Escherichia coli ?
-
 ?
S-methyl-L-Cys + H2O
-
 Escherichia coli ?
-
 ?
S-methyl-L-Cys + H2O
-
 Providencia rettgeri ?
-
 ?
S-methyl-L-Cys + indole
-
 Escherichia coli Trp + ?
-
 ?
Ser + H2O
-
 Escherichia coli ?
-
 ?
Ser + H2O
-
 Providencia rettgeri ?
-
 ?
Ser + indole
-
 Escherichia coli Trp + ?
-
 ?

Subunits

Subunits Comment Organism
More the dimeric form is not active Escherichia coli
tetramer
-
 Aeromonas hydrophila
tetramer
-
 Providencia rettgeri
tetramer
-
 Paenibacillus alvei
tetramer 4 * ? Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2 8.8 strain B/1t7-A Escherichia coli
7.5 8.4
-
 Paenibacillus alvei
8 9
-
 Providencia rettgeri
8.2
-
-
 Aeromonas hydrophila

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Vibrio sp.
pyridoxal 5'-phosphate some coenzyme analogues can replace pyridoxal 5'-phosphate Escherichia coli
pyridoxal 5'-phosphate one enzyme molecule contains 4 pyridoxal 5'-phosphate combining sites Escherichia coli
pyridoxal 5'-phosphate one enzyme molecule contains 4 pyridoxal 5'-phosphate combining sites Aeromonas hydrophila
pyridoxal 5'-phosphate one enzyme molecule contains 4 pyridoxal 5'-phosphate combining sites Providencia rettgeri
pyridoxal 5'-phosphate one enzyme molecule contains 4 pyridoxal 5'-phosphate combining sites Paenibacillus alvei