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Literature summary for 4.1.99.1 extracted from

  • Behbahani-Nejad, I.; Dye, J.L.; Suelter, C.H.
    Tryptophanase from Escherichia coli B/1t7-A (1987), Methods Enzymol., 142, 414-422.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.019
-
indole
-
Escherichia coli
0.06
-
S-o-nitrophenyl-L-Cys
-
Escherichia coli
0.067
-
S-benzylcysteine
-
Escherichia coli
0.33
-
L-Trp
-
Escherichia coli
1.2
-
beta-chloroalanine
-
Escherichia coli
11
-
Cys
-
Escherichia coli
12
-
S-methyl-L-Cys
-
Escherichia coli
70
-
pyruvate
-
Escherichia coli
160
-
L-Ser strain B/1t7-A, 37°C Escherichia coli
345
-
NH4+
-
Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
K+ monovalent cation activator required Escherichia coli
NH4+ monovalent cation activator required Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
220000
-
-
Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli B/1t7-A
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
50
-
-
Escherichia coli

Storage Stability

Storage Stability Organism
stored as a suspension under nitrogen in 0.1 M potassium phosphate, pH 8.0, 0.2 mM dithiothreitol, 1 mM EDTA, and 2 M (NH4)2SO4 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-chloroalanine + H2O
-
Escherichia coli ?
-
?
beta-chloroalanine + H2O
-
Escherichia coli B/1t7-A ?
-
?
Cys + H2O
-
Escherichia coli ?
-
?
L-Trp + H2O
-
Escherichia coli indole + pyruvate + NH4+
-
?
S-benzylcysteine + H2O
-
Escherichia coli ?
-
?
S-benzylcysteine + H2O
-
Escherichia coli B/1t7-A ?
-
?
S-methyl-L-Cys + indole
-
Escherichia coli Trp + ?
-
?
S-methyl-L-Cys + indole
-
Escherichia coli B/1t7-A Trp + ?
-
?
S-o-nitrophenyl-L-Cys + H2O
-
Escherichia coli o-nitrothiophenol + pyruvate + NH3
-
?
S-o-nitrophenyl-L-Cys + H2O
-
Escherichia coli B/1t7-A o-nitrothiophenol + pyruvate + NH3
-
?
Ser + indole
-
Escherichia coli Trp + ?
-
?
Ser + indole
-
Escherichia coli B/1t7-A Trp + ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * ? Escherichia coli

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate dependent on Escherichia coli
pyridoxal 5'-phosphate binds 1 mol of pyridoxal 5'-phosphate per subunit Escherichia coli