Literature summary for 4.1.3.6 extracted from

Hu, Y.; Holden, J.F.
Citric acid cycle in the hyperthermophilic archaeon Pyrobaculum islandicum grown autotrophically, heterotrophically, and mixotrophically with acetate (2006), J. Bacteriol., 188, 4350-4355.

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Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
citrate	Pyrobaculum islandicum	work opposite to the citrate synthase reaction, involved in the control of the direction of carbon flow in the citric acid cycle	acetate + oxaloacetate	-	?

Organism

Organism	UniProt	Comment	Textmining
Pyrobaculum islandicum	-	strain DSM 4184, grown at 95°C autotrophically, heterotrophically, and mixotrophically with acetate, H2, and small amounts of yeast extract and with thiosulfate as the terminal electron acceptor	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
additional information	citrate lyase activity observed only when an acetylating compound is present in the assay mixture, acetylation possibly involved in the regulation of activity	Pyrobaculum islandicum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.003	-	tryptone and yeast extract as growth substrates	Pyrobaculum islandicum
0.009	-	H2, acetate and yeast extract as growth substrates	Pyrobaculum islandicum
0.025	-	H2 and CO2 as growth substrates	Pyrobaculum islandicum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
citrate	work opposite to the citrate synthase reaction, involved in the control of the direction of carbon flow in the citric acid cycle	Pyrobaculum islandicum	acetate + oxaloacetate	-	?

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Release 2023.1 (January 2023)