Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 100 mM KH2PO4/Na2HPO4 pH 6.2, 10% (w/v) PEG 3000 | Methanocaldococcus jannaschii |
Protein Variants | Comment | Organism |
---|---|---|
C195S | the mutant shows reduced activity compared tot he wild type enzyme | Methanocaldococcus jannaschii |
K41A | inactive | Methanocaldococcus jannaschii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N1-methylguanine37 in tRNAPhe + pyruvate + S-adenosyl-L-methionine | Methanocaldococcus jannaschii | - |
4-demethylwyosine37 in tRNAPhe + L-methionine + 5'-deoxyadenosine + CO2 + H2O | - |
ir | |
N1-methylguanine37 in tRNAPhe + pyruvate + S-adenosyl-L-methionine | Methanocaldococcus jannaschii DSM 2661 | - |
4-demethylwyosine37 in tRNAPhe + L-methionine + 5'-deoxyadenosine + CO2 + H2O | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | Q57705 | - |
- |
Methanocaldococcus jannaschii DSM 2661 | Q57705 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, butyl Sepharose column chromatography, and HiTrap chelating column chromatography | Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N1-methylguanine37 in tRNAPhe + pyruvate + S-adenosyl-L-methionine | - |
Methanocaldococcus jannaschii | 4-demethylwyosine37 in tRNAPhe + L-methionine + 5'-deoxyadenosine + CO2 + H2O | - |
ir | |
N1-methylguanine37 in tRNAPhe + pyruvate + S-adenosyl-L-methionine | - |
Methanocaldococcus jannaschii DSM 2661 | 4-demethylwyosine37 in tRNAPhe + L-methionine + 5'-deoxyadenosine + CO2 + H2O | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
TYW1 | - |
Methanocaldococcus jannaschii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Methanocaldococcus jannaschii | |
[4Fe-4S]-center | - |
Methanocaldococcus jannaschii |