Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pyrococcus abyssi |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | protein contains contained up to 8.2 iron and 8.2 sulfide ions per polypeptide chain | Pyrococcus abyssi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
x * 37800, calculated, x * 37000, SDS-PAGE | Pyrococcus abyssi |
37800 | - |
x * 37800, calculated, x * 37000, SDS-PAGE | Pyrococcus abyssi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus abyssi | Q9V1F9 | - |
- |
Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543 | Q9V1F9 | - |
- |
Purification (Comment) | Organism |
---|---|
purification and reconstitution of recombinant protein | Pyrococcus abyssi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N1-methylguanine37 in tRNAPhe + pyruvate + S-adenosyl-L-methionine | - |
Pyrococcus abyssi | 4-demethylwyosine37 in tRNAPhe + L-methionine + 5'-deoxyadenosine + CO2 + H2O | - |
? | |
N1-methylguanine37 in tRNAPhe + pyruvate + S-adenosyl-L-methionine | - |
Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543 | 4-demethylwyosine37 in tRNAPhe + L-methionine + 5'-deoxyadenosine + CO2 + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37800, calculated, x * 37000, SDS-PAGE | Pyrococcus abyssi |
Synonyms | Comment | Organism |
---|---|---|
TYW1 | - |
Pyrococcus abyssi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
assay at | Pyrococcus abyssi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Pyrococcus abyssi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
[4Fe-4S]-center | the N-terminal half of the enzyme beside the radical-SAM cysteine triad, contains an additional highly conserved cysteine motif involved in the coordination of a second [4Fe-4S] cluster. This cluster displays a free coordination site that interacts with pyruvate, the second substrate of the reaction | Pyrococcus abyssi |