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Literature summary for 4.1.3.44 extracted from

  • Perche-Letuvee, P.; Kathirvelu, V.; Berggren, G.; Clemancey, M.; Latour, J.M.; Maurel, V.; Douki, T.; Armengaud, J.; Mulliez, E.; Fontecave, M.; Garcia-Serres, R.; Gambarelli, S.; Atta, M.
    4-Demethylwyosine synthase from Pyrococcus abyssi is a radical-S-adenosyl-L-methionine enzyme with an additional [4Fe-4S](+2) cluster that interacts with the pyruvate co-substrate (2012), J. Biol. Chem., 287, 41174-41185.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.3.44

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pyrococcus abyssi

Metals/Ions

Metals/Ions Comment Organism Structure
Iron protein contains contained up to 8.2 iron and 8.2 sulfide ions per polypeptide chain Pyrococcus abyssi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
 x * 37800, calculated, x * 37000, SDS-PAGE Pyrococcus abyssi
37800
-
 x * 37800, calculated, x * 37000, SDS-PAGE Pyrococcus abyssi

Organism

Organism UniProt Comment Textmining
Pyrococcus abyssi Q9V1F9
-
-
Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543 Q9V1F9
-
-

Purification (Commentary)

Purification (Comment) Organism
purification and reconstitution of recombinant protein Pyrococcus abyssi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N1-methylguanine37 in tRNAPhe + pyruvate + S-adenosyl-L-methionine
-
 Pyrococcus abyssi 4-demethylwyosine37 in tRNAPhe + L-methionine + 5'-deoxyadenosine + CO2 + H2O
-
 ?
N1-methylguanine37 in tRNAPhe + pyruvate + S-adenosyl-L-methionine
-
 Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543 4-demethylwyosine37 in tRNAPhe + L-methionine + 5'-deoxyadenosine + CO2 + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 37800, calculated, x * 37000, SDS-PAGE Pyrococcus abyssi

Synonyms

Synonyms Comment Organism
TYW1
-
 Pyrococcus abyssi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 assay at Pyrococcus abyssi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Pyrococcus abyssi

Cofactor

Cofactor Comment Organism Structure
[4Fe-4S]-center the N-terminal half of the enzyme beside the radical-SAM cysteine triad, contains an additional highly conserved cysteine motif involved in the coordination of a second [4Fe-4S] cluster. This cluster displays a free coordination site that interacts with pyruvate, the second substrate of the reaction Pyrococcus abyssi