Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.3.43 extracted from

  • Carere, J.; Baker, P.; Seah, S.Y.K.
    Investigating the molecular determinants for substrate channeling in BphI-BphJ, an aldolase-dehydrogenase complex from the polychlorinated biphenyls degradation pathway (2011), Biochemistry, 50, 8407-8416.
    View publication on PubMed
Search for more literature for 4.1.3.43

Cloned(Commentary)

Cloned (Comment) Organism
bphI and bphJ cloned into the plasmids pBTL4-T7 and pET28a Paraburkholderia xenovorans

Protein Variants

Protein Variants Comment Organism
G322A channels acetaldehyde with similar efficiency to wild-type, 30% lowered efficiency of isobutyraldehyde channeling Paraburkholderia xenovorans
G322F unable to channel either acetaldehyde or propionaldehyde Paraburkholderia xenovorans
G322L unable to channel either acetaldehyde or propionaldehyde Paraburkholderia xenovorans
G323A channels acetaldehyde with similar efficiency to wild-type, unable to channel isobutyraldehyde Paraburkholderia xenovorans
G323F unable to channel either acetaldehyde or propionaldehyde Paraburkholderia xenovorans
G323L 63% channeling efficiency for acetaldehyde, unable to channel propionaldehyde Paraburkholderia xenovorans
H20A reduction of acetaldehyde and propionaldehyde channeling by more than 70% Paraburkholderia xenovorans
L89A reduction in channeling efficiency of 30% for all aldehydes tested Paraburkholderia xenovorans
additional information all mutations created by site specific mutagenesis Paraburkholderia xenovorans
Y290F reduction in channeling efficiency of 30% for all aldehydes tested Paraburkholderia xenovorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.053
-
 4-hydroxy-2-oxopentanoate G322F mutant, steady state, absence of BphJ cofactors, pH 8.0, 25°C Paraburkholderia xenovorans
0.06
-
 4-hydroxy-2-oxopentanoate G323A mutant, steady state, pH 8.0, 25°C Paraburkholderia xenovorans
0.081
-
 4-hydroxy-2-oxopentanoate G322L mutant, steady state, absence of BphJ cofactors, pH 8.0, 25°C Paraburkholderia xenovorans
0.089
-
 4-hydroxy-2-oxopentanoate wild-type protein, steady state, pH 8.0, 25°C Paraburkholderia xenovorans
0.09
-
 4-hydroxy-2-oxopentanoate G322F mutant, steady state, pH 8.0, 25°C Paraburkholderia xenovorans
0.117
-
 4-hydroxy-2-oxohexanoate wild-type protein, steady state, pH 8.0, 25°C Paraburkholderia xenovorans
0.13
-
 4-hydroxy-2-oxopentanoate G323F mutant, steady state, absence of BphJ cofactors, pH 8.0, 25°C Paraburkholderia xenovorans
0.14
-
 4-hydroxy-2-oxopentanoate G322L mutant, steady state, pH 8.0, 25°C Paraburkholderia xenovorans
0.158
-
 4-hydroxy-2-oxopentanoate wild-type protein, steady state, absence of BphJ cofactors, pH 8.0, 25°C Paraburkholderia xenovorans
0.16
-
 4-hydroxy-2-oxopentanoate G323L mutant, steady state, pH 8.0, 25°C Paraburkholderia xenovorans
0.18
-
 4-hydroxy-2-oxopentanoate G322A mutant, steady state, pH 8.0, 25°C Paraburkholderia xenovorans
0.216
-
 4-hydroxy-2-oxopentanoate G323L mutant, steady state, absence of BphJ cofactors, pH 8.0, 25°C Paraburkholderia xenovorans
0.3
-
 4-hydroxy-2-oxohexanoate G322F mutant, steady state, pH 8.0, 25°C Paraburkholderia xenovorans
0.35
-
 4-hydroxy-2-oxohexanoate G322L mutant, steady state, pH 8.0, 25°C Paraburkholderia xenovorans
0.36
-
 4-hydroxy-2-oxohexanoate G323A mutant, steady state, pH 8.0, 25°C Paraburkholderia xenovorans
0.37
-
 4-hydroxy-2-oxohexanoate G323L mutant, steady state, pH 8.0, 25°C Paraburkholderia xenovorans
0.52
-
 4-hydroxy-2-oxohexanoate G322A mutant, steady state, pH 8.0, 25°C Paraburkholderia xenovorans
0.94
-
 4-hydroxy-2-oxopentanoate G323F mutant, steady state, pH 8.0, 25°C Paraburkholderia xenovorans
1.1
-
 4-hydroxy-2-oxohexanoate G323F mutant, steady state, pH 8.0, 25°C Paraburkholderia xenovorans

Organism

Organism UniProt Comment Textmining
Paraburkholderia xenovorans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
BphI and BphJ form a stable complex as they bind and coelute from Ni2+-NTA column, after purification N-terminal histidine tag of BphJ is proteolytically cleaved by thrombin digestion Paraburkholderia xenovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroxy-2-oxohexanoate 4-hydroxy-2-oxohexanoate i.e. HOHA Paraburkholderia xenovorans propionaldehyde + pyruvate
-
 ?
4-hydroxy-2-oxopentanoate 4-hydroxy-2-oxopentanoate i.e. HOPA Paraburkholderia xenovorans acetaldehyde + pyruvate
-
 ?

Synonyms

Synonyms Comment Organism
BphI
-
 Paraburkholderia xenovorans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
 4-hydroxy-2-oxopentanoate G323L mutant, steady state, absence of BphJ cofactors, pH 8.0, 25°C Paraburkholderia xenovorans
0.27
-
 4-hydroxy-2-oxohexanoate G323L mutant, steady state, pH 8.0, 25°C Paraburkholderia xenovorans
0.286
-
 4-hydroxy-2-oxopentanoate G322L mutant, steady state, absence of BphJ cofactors, pH 8.0, 25°C Paraburkholderia xenovorans
0.34
-
 4-hydroxy-2-oxopentanoate G323A mutant, steady state, pH 8.0, 25°C Paraburkholderia xenovorans
0.45
-
 4-hydroxy-2-oxopentanoate G322F mutant, steady state, absence of BphJ cofactors, pH 8.0, 25°C Paraburkholderia xenovorans
0.46
-
 4-hydroxy-2-oxohexanoate G323A mutant, steady state, pH 8.0, 25°C Paraburkholderia xenovorans
0.64
-
 4-hydroxy-2-oxopentanoate G323L mutant, steady state, pH 8.0, 25°C Paraburkholderia xenovorans
0.72
-
 4-hydroxy-2-oxopentanoate G323F mutant, steady state, absence of BphJ cofactors, pH 8.0, 25°C Paraburkholderia xenovorans
0.79
-
 4-hydroxy-2-oxopentanoate wild-type protein, steady state, absence of BphJ cofactors, pH 8.0, 25°C Paraburkholderia xenovorans
0.94
-
 4-hydroxy-2-oxohexanoate G322L mutant, steady state, pH 8.0, 25°C Paraburkholderia xenovorans
1.05
-
 4-hydroxy-2-oxohexanoate G322F mutant, steady state, pH 8.0, 25°C Paraburkholderia xenovorans
1.06
-
 4-hydroxy-2-oxopentanoate G322F mutant, steady state, pH 8.0, 25°C Paraburkholderia xenovorans
1.09
-
 4-hydroxy-2-oxopentanoate G322L mutant, steady state, pH 8.0, 25°C Paraburkholderia xenovorans
1.3
-
 4-hydroxy-2-oxohexanoate G323F mutant, steady state, pH 8.0, 25°C Paraburkholderia xenovorans
1.9
-
 4-hydroxy-2-oxohexanoate G322A mutant, steady state, pH 8.0, 25°C Paraburkholderia xenovorans
2.4
-
 4-hydroxy-2-oxopentanoate G322A mutant, steady state, pH 8.0, 25°C Paraburkholderia xenovorans
3.9
-
 4-hydroxy-2-oxohexanoate wild-type protein, steady state, pH 8.0, 25°C Paraburkholderia xenovorans
4.07
-
 4-hydroxy-2-oxopentanoate wild-type protein, steady state, pH 8.0, 25°C Paraburkholderia xenovorans
4.3
-
 4-hydroxy-2-oxopentanoate G323F mutant, steady state, pH 8.0, 25°C Paraburkholderia xenovorans

General Information

General Information Comment Organism
metabolism enzyme is part of the BphI-BphJ-complex, an aldolase-dehydrogenase complex from the polychlorinated biphenyl degradation pathway Paraburkholderia xenovorans