Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Bromopyruvate | 85% inhibition at 1 mM, pyruvate or 2-keto-4-hydroxyglutarate protect; substrate analog. Treatment results in a time- and concentration-dependent loss of enzymatic activity. The substrates pyruvate and 2-keto-4-hydroxyglutarate provide more than 90% protection against inactivation by bromopyruvate, no protective effect is seen with glycolaldehyde. 1.1 mol of bromopyruvate is incorporated per enzyme subunit by esterification of residue Glu45 | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli | P0A955 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | active-site residues | Escherichia coli | ? | - |
? | |
additional information | residue Glu45 is essential for catalytic activity, most likely acting as the amphoteric proton donor/acceptor | Escherichia coli | ? | - |
? |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
1.6 | - |
95% loss of activity after 10 min at 4°C, reactivation within 20 min at pH 6-8 | Escherichia coli |