General Stability | Organism |
---|---|
irreversibly inactivated by CN- in presence of 4-hydroxy-2-oxoglutarate | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-mercuribenzenesulfonic acid | 1 mM, 56% inhibition | Escherichia coli | |
CN- | irreversible, in presence of 4-hydroxy-2-oxoglutarate; irreversible loss of activity in presence of glyoxylate, but not in presence of pyruvate | Escherichia coli | |
iodoacetate | 46% inhibition at 5 mM; 50 mM, almost complete inhibition | Escherichia coli | |
additional information | not inhibitory: EDTA, 1,10-phenanthroline, S-hydroxyquinoline, or alpha,alpha'-dipyridyl | Escherichia coli | |
N-ethylmaleimide | 10% inhibition at 2.5 mM | Escherichia coli | |
NaBH4 | extensive loss of activity after incubation of enzyme with NaBH4 in presence of pyruvate or glyoxylate | Escherichia coli | |
p-Mercuriphenylsulfonate | 50% inhibition at 0.5 mM | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Escherichia coli | |
2.3 | - |
(L)-4-hydroxy-alpha-ketoglutarate | - |
Escherichia coli | |
2.3 | - |
(4S)-4-hydroxy-2-oxoglutarate | pH 8.1, 25°C | Escherichia coli | |
2.5 | - |
(D)-4-hydroxy-alpha-ketoglutarate | - |
Escherichia coli | |
25 | - |
(4R)-4-hydroxy-2-oxoglutarate | pH 8.1, 25°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement | Escherichia coli | |
additional information | divalent kations are not required. No effect on activity: Mg2+, Mn2+, Zn2+, Ca2+, Ba2+, Co2+, Cu2+, Ni2+, Fe2+, Hg2+, K+, or Fe3+ | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62500 | - |
gel filtration | Escherichia coli |
64000 | - |
sucrose density gradient centrifugation | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxy-2-oxoglutarate | Escherichia coli | possibly involved in regulation of tricarboxylic acid cycle | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli | P0A955 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.53 | - |
(D)-4-hydroxy-alpha-ketoglutarate | Escherichia coli |
7.9 | - |
(L)-4-hydroxy-alpha-ketoglutarate | Escherichia coli |
96.7 | - |
pH 8.1, 25°C | Escherichia coli |
Storage Stability | Organism |
---|---|
4¦C, Tris-HCl buffer, pH 7.4, 40-50% loss of activity after 1 month, more labile when frozen | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(4R)-4-hydroxy-2-oxoglutarate | poor substrate | Escherichia coli | pyruvate + glyoxylate | - |
r | |
(4S)-4-hydroxy-2-oxobutanoate | at 8% of the rate with DL-4-hydroxy-2-oxoglutarate | Escherichia coli | formaldehyde | - |
r | |
(4S)-4-hydroxy-2-oxoglutarate | - |
Escherichia coli | pyruvate + glyoxylate | L-4-hydroxy-2-oxoglutarate, pyruvate and glyoxylate all bind at the same active site of the enzyme | r | |
2-Keto-4-hydroxybutyrate | - |
Escherichia coli | Pyruvate + formaldehyde | - |
? | |
4-Hydroxy-2-oxoglutarate | - |
Escherichia coli | Pyruvate + glyoxylate | - |
? | |
4-hydroxy-2-oxoglutarate | possibly involved in regulation of tricarboxylic acid cycle | Escherichia coli | ? | - |
? | |
Glyoxylate + pyruvate | higher specificity for pyruvate than for glyoxylate | Escherichia coli | 4-Hydroxy-2-ketoglutarate | - |
? | |
additional information | highly specific towards L-isomer | Escherichia coli | ? | - |
? | |
additional information | enzyme additionally shows beta-decarboxylase activity towards oxalacetate | Escherichia coli | ? | - |
? | |
Oxaloacetate | better carboxylase than aldolase | Escherichia coli | CO2 + pyruvate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
enzyme assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
2.2 to 2.4 times higher activity is measured in Tris-HCl buffer than in potassium phosphate buffer at the same pH values | Escherichia coli |
8.6 | - |
- |
Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
p-Mercuriphenylsulfonate | - |
Escherichia coli |