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Literature summary for 4.1.3.4 extracted from
- Pseudomonas mevalonii 3-hydroxy-3-methylglutaryl-CoA lyase (2000), Methods Enzymol., 324, 139-149.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | activates 2fold at 5 mM | Pseudomonas mevalonii |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Pseudomonas mevalonii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (R)-3-hydroxy-3-methylglutaryl-CoA | - |
Pseudomonas mevalonii | |
| 1,10-phenanthroline | 43% activity after 4 h at 4 mM | Pseudomonas mevalonii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
(S)-3-hydroxy-3-methylglutaryl-CoA | pH 8.2, 30°C, recombinant enzyme | Pseudomonas mevalonii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | tightly bound to the enzyme | Pseudomonas mevalonii | |
| Mg2+ | increases activity at millimolar concentrations | Pseudomonas mevalonii | |
| Mn2+ | increases activity at micromolar concentrations | Pseudomonas mevalonii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 64000 | - |
calculated from Stokes' radius | Pseudomonas mevalonii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Pseudomonas mevalonii | last step in ketogenesis | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas mevalonii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas mevalonii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Pseudomonas mevalonii |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, retains full activity for several months in phosphate buffer containing 20% glycerol at pH 7.2 | Pseudomonas mevalonii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-3-Hydroxy-3-methylglutaryl-CoA | - |
Pseudomonas mevalonii | Acetyl-CoA + acetoacetate | - |
ir | |
| additional information | last step in ketogenesis | Pseudomonas mevalonii | ? | - |
? | |
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