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Literature summary for 4.1.3.4 extracted from
- Evaluation of 3-hydroxy-3-methylglutaryl-coenzyme A lyase arginine-41 as a catalytic residue: Use of acetyldithio-coenzyme A to monitor product enolization (2004), Biochemistry, 43, 5287-5295.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D42A | 4fold decrease in proton exchange | Homo sapiens |
| H233A | 10fold decrease in proton exchange | Homo sapiens |
| R41M | lower activity than R41Q mutant | Homo sapiens |
| R41Q | mutation leads to human HMG-CoA lyase deficiency | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetoacetate | - |
Homo sapiens |  |
| acetyldithio-CoA | - |
Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 48 | - |
(S)-3-hydroxy-3-methylglutaryl-CoA | wild-type | Homo sapiens | |
| 62 | - |
(S)-3-hydroxy-3-methylglutaryl-CoA | R41Q mutant | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required, Km wild-type enzyme: 0.2mM, Km R41Q mutant: 0.088 mM | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Homo sapiens | last step in ketogenesis | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-3-Hydroxy-3-methylglutaryl-CoA | - |
Homo sapiens | Acetyl-CoA + acetoacetate | - |
? | |
| additional information | last step in ketogenesis | Homo sapiens | ? | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0004 | - |
(S)-3-hydroxy-3-methylglutaryl-CoA | R41Q mutant | Homo sapiens | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.021 | - |
acetoacetate | - |
Homo sapiens | |
| 0.17 | - |
acetyldithio-CoA | - |
Homo sapiens | |
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Release 2023.1 (January 2023)
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