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Literature summary for 4.1.3.4 extracted from
- Pseudomonas mevalonii 3-hydroxy-3-methylglutaryl-CoA lyase: characterization of the isolated recombinant protein and investigation of the enzyme's cation requirements (1992), Biochemistry, 31, 11224-11230.
General Stability
| General Stability | Organism |
|---|---|
| extremely unstable, even in presence of glycerol | Pseudomonas mevalonii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | not | Pseudomonas mevalonii |  |
| o-phenanthroline | not m-Phenanthroline, probably acts as metal chelator | Pseudomonas mevalonii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
hydroxymethylglutaryl-CoA | wild-type | Pseudomonas mevalonii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Bivalent cations | stimulate | Pseudomonas mevalonii | |
| Cu2+ | tightly bound, stoichiometry | Pseudomonas mevalonii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
x * 32000, SDS-PAGE | Pseudomonas mevalonii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas mevalonii | - |
recombinant enzyme | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| overexpressed in Escherichia coli, immunoreaction with avian liver enzyme antibodies | Pseudomonas mevalonii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Pseudomonas mevalonii |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, phosphate buffer, pH 7.2, 20% glycerol, several weeks | Pseudomonas mevalonii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-hydroxy-3-methylglutaryl-CoA | - |
Pseudomonas mevalonii | acetoacetate + acetyl-CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 32000, SDS-PAGE | Pseudomonas mevalonii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
enzyme assay at | Pseudomonas mevalonii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.2 | - |
enzyme assay at | Pseudomonas mevalonii |
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