Activating Compound | Comment | Organism | Structure |
---|---|---|---|
BphJ | the aldehyde dehydrogenase BphJ coordinates the catalytic activity of BphI through allostery rather than through aldehyde channeling | Paraburkholderia xenovorans |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Paraburkholderia xenovorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.089 | - |
(S)-4-hydroxy-2-oxopentanoate | in the presence of NADH, in 100 mM HEPES pH 8.0, at 25°C | Paraburkholderia xenovorans | |
0.158 | - |
(S)-4-hydroxy-2-oxopentanoate | in the absence of NADH, in 100 mM HEPES pH 8.0, at 25°C | Paraburkholderia xenovorans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paraburkholderia xenovorans | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-4-hydroxy-2-oxopentanoate | - |
Paraburkholderia xenovorans | ethanal + pyruvate | - |
? | |
additional information | BphJ forms a heterotetrameric complex with BphI that channels aldehydes produced in the aldol cleavage reaction to the dehydrogenase via a molecular tunnel | Paraburkholderia xenovorans | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BphI | - |
Paraburkholderia xenovorans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.79 | - |
(S)-4-hydroxy-2-oxopentanoate | in the absence of NADH, in 100 mM HEPES pH 8.0, at 25°C | Paraburkholderia xenovorans | |
4.07 | - |
(S)-4-hydroxy-2-oxopentanoate | in the presence of NADH, in 100 mM HEPES pH 8.0, at 25°C | Paraburkholderia xenovorans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | BphI is activated in the presence of the BphJ cofactor, NADH, by about 5fold | Paraburkholderia xenovorans |