Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis of the gene nahM from the naphthalene catabolic plasmid pWW60-22 of strain NCIMB9816, gene nahM is organized in the nahNLOMK operon of nahOH genes, genetic organization analysis, overview, overexpression in Escherichia coli strain BL21(DE3) using different plasmid vectors, overview | Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | - |
Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxy-2-oxovalerate | Pseudomonas putida | step in a meta pathway | pyruvate + acetaldehyde | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | P51017 | nahKLMO operon; wild-type strain NCIMB9816, gene nahM | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.015 | 2.3 | recombinant enzyme in extracts of different recombinant strains, activity depends on the plasmid vector used for expression and the aldehyde dehydrogenase, EC 1.2.1.10, overview | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxy-2-oxovalerate | step in a meta pathway | Pseudomonas putida | pyruvate + acetaldehyde | - |
? | |
4-hydroxy-2-oxovalerate | enzyme acts stereospecifically on the L-enantiomer | Pseudomonas putida | pyruvate + acetaldehyde | - |
? | |
additional information | the enzyme acts in an enzyme complex with the aldehyde dehydrogenase, EC 1.2.1.10, which acts on acetaldehyde to form acetyl-CoA | Pseudomonas putida | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HOA | - |
Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Pseudomonas putida |