Cloned (Comment) | Organism |
---|---|
cloned, sequenced and expressed in Escherichia coli BL21(DE3) | Hydrogenobacter thermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Hydrogenobacter thermophilus | |
citrate | - |
Hydrogenobacter thermophilus | |
CoA | - |
Hydrogenobacter thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
acetyl-CoA | pH 8.0, 70°C | Hydrogenobacter thermophilus | |
0.082 | - |
citryl-CoA | pH 8.0, 70°C | Hydrogenobacter thermophilus | |
0.13 | - |
oxaloacetate | pH 8.0, 70°C | Hydrogenobacter thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
3 * 30000, homotrimer, SDS-PAGE | Hydrogenobacter thermophilus |
87000 | - |
gel filtration | Hydrogenobacter thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3S)-citryl-CoA | Hydrogenobacter thermophilus | reductive tricarboxylic acid cycle, second step of citrate cleavage reaction | acetyl-CoA + oxaloacetate | - |
r | |
(3S)-citryl-CoA | Hydrogenobacter thermophilus TK-6 / IAM 12695 | reductive tricarboxylic acid cycle, second step of citrate cleavage reaction | acetyl-CoA + oxaloacetate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hydrogenobacter thermophilus | - |
- |
- |
Hydrogenobacter thermophilus TK-6 / IAM 12695 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native and recombinant enzyme | Hydrogenobacter thermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1 | - |
citrate synthase activity as a side reaction | Hydrogenobacter thermophilus |
33 | - |
citrate, ATP, CoA and citryl-CoA forming enzyme CCS in the enzyme assay | Hydrogenobacter thermophilus |
280 | - |
purified citryl-CoA as substrate | Hydrogenobacter thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3S)-citryl-CoA | - |
Hydrogenobacter thermophilus | acetyl-CoA + oxaloacetate | - |
r | |
(3S)-citryl-CoA | reductive tricarboxylic acid cycle, second step of citrate cleavage reaction | Hydrogenobacter thermophilus | acetyl-CoA + oxaloacetate | - |
r | |
(3S)-citryl-CoA | - |
Hydrogenobacter thermophilus TK-6 / IAM 12695 | acetyl-CoA + oxaloacetate | - |
r | |
(3S)-citryl-CoA | reductive tricarboxylic acid cycle, second step of citrate cleavage reaction | Hydrogenobacter thermophilus TK-6 / IAM 12695 | acetyl-CoA + oxaloacetate | - |
r | |
additional information | shows low citrate synthase activity | Hydrogenobacter thermophilus | ? | - |
? | |
additional information | shows low citrate synthase activity | Hydrogenobacter thermophilus TK-6 / IAM 12695 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 30000, homotrimer, SDS-PAGE | Hydrogenobacter thermophilus |
Synonyms | Comment | Organism |
---|---|---|
CCL | - |
Hydrogenobacter thermophilus |
citryl-CoA lyase | - |
Hydrogenobacter thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | 90 | thermostable with no detectable loss of activity at 80°C for 10 min, retains 83% after 90°C for 10 min | Hydrogenobacter thermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
570 | - |
citryl-CoA | pH 8.0, 70°C | Hydrogenobacter thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | - |
Hydrogenobacter thermophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9.5 | 10% of maximum activity at pH 7.0, 50% of maximum activity at pH 7.5, 90% of maximum activity at pH 9.0, 30% of maximum activity at pH 9.5 | Hydrogenobacter thermophilus |