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Literature summary for 4.1.3.30 extracted from
- Purification and properties of methylisocitrate lyase, a key enzyme in propionate metabolism, from Candida lipolytica (1977), Agric. Biol. Chem., 41, 169-174.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| sulfhydryl compound | required for optimal activity, Km for Cys: 0.43 mM | Yarrowia lipolytica |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 10 mM, in presence of 2.5 mM Mg2+, 10% inhibition | Yarrowia lipolytica | |
| H2O2 | - |
Yarrowia lipolytica | |
| Hg2+ | 10 mM, in presence of 2.5 mM Mg2+, 30% inhibition | Yarrowia lipolytica | |
| iodoacetate | - |
Yarrowia lipolytica | |
| NADH | noncompetitive | Yarrowia lipolytica | |
| NADPH | noncompetitive | Yarrowia lipolytica | |
| p-chloromercuribenzoate | - |
Yarrowia lipolytica | |
| Pb2+ | 10 mM, in presence of 2.5 mM Mg2+, 14% inhibition. 11% activation, when added instead of Mg2+ | Yarrowia lipolytica | |
| sulfhydryl reagents | - |
Yarrowia lipolytica | |
| Zn2+ | 10 mM, in presence of 2.5 mM Mg2+, 34% inhibition | Yarrowia lipolytica | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.77 | - |
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate | - |
Yarrowia lipolytica | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | divalent cation required, Mg2+ most effective, Km: 0.6 mM | Yarrowia lipolytica | |
| Mn2+ | 48% of the activition with Mg2+ | Yarrowia lipolytica | |
| Pb2+ | 11% activation, when added instead of Mg2+. 10 mM, in presence of 2.5 mM Mg2+, 14% inhibition | Yarrowia lipolytica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 120000 | - |
gel filtration | Yarrowia lipolytica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate | Yarrowia lipolytica | key enzyme in propionate metabolism | ? | - |
? | |
| (2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate | Yarrowia lipolytica | regulatory enzyme of the methylcitric acid cycle | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Yarrowia lipolytica | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Yarrowia lipolytica |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.76 | - |
- |
Yarrowia lipolytica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate | reverse reaction not detected | Yarrowia lipolytica | Pyruvate + succinate | - |
? | |
| (2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate | specific for threo-DS-2-methylisocitrate | Yarrowia lipolytica | Pyruvate + succinate | - |
? | |
| (2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate | no activity with 2R,3S-isocitrate | Yarrowia lipolytica | Pyruvate + succinate | - |
? | |
| (2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate | key enzyme in propionate metabolism | Yarrowia lipolytica | ? | - |
? | |
| (2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate | regulatory enzyme of the methylcitric acid cycle | Yarrowia lipolytica | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Yarrowia lipolytica |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 50 | 20°C: about 50% of maximal activity, 50°C: about 30% of maximal activity | Yarrowia lipolytica |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
10 min, fairly stable | Yarrowia lipolytica |
| 60 | - |
10 min, complete loss of activity | Yarrowia lipolytica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.7 | - |
Yarrowia lipolytica |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8.5 | pH 6.5: about 60% of maximal activity, pH 8.5: about 35% of maximal activity | Yarrowia lipolytica |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
15°C, 20 h, fairly stable | Yarrowia lipolytica |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | activates | Yarrowia lipolytica | |
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