Cloned (Comment) | Organism |
---|---|
gene nal, co-expression in Escherichia coli strain Rosetta (DE3) pLysS with N-acetylglucosamine 2-epimerase from Synechocystis sp. PCC 6803 (snAGE) or Anabaena sp. CH1 (anAGE) | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | synthesis of N-acetylneuraminic acid from N-acetylglucosamine and pyruvate by constructing and co-expressing a polycistronic plasmid encoding an N-acetylglucosamine 2-epimerase gene from Synechocystis sp. PCC 6803 (snAGE) or Anabaena sp. CH1 (anAGE) and the Escherichia coli N-acetylneuraminic acid lyase gene. Construction of four polycistronic plasmids in which the positions of AGE gene differ with respect to Nal gene, plasmid pET-28a-Nal-anAGE with anAGE gene located next to Nal gene produces the highest amount of N-acetylneuraminic acid, overview. anAGE from Anabaena sp. CH1 shows much higher activity and productivity than snAGE from Synechocystis sp. PCC 6803 | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Synonyms | Comment | Organism |
---|---|---|
N-Acetylneuraminic acid lyase | - |
Escherichia coli |
NAL | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |