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Literature summary for 4.1.3.27 extracted from

  • Barone, P.; Zhang, X.H.; Widholm, J.M.
    Tobacco plastid transformation using the feedback-insensitive anthranilate synthase [alpha]-subunit of tobacco (ASA2) as a new selectable marker (2009), J. Exp. Bot., 60, 3195-3202.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.3.27

Application

Application Comment Organism
biotechnology development of ASA2 as a chloroplast selective marker, which is important not only for biosafety reasons, but also for practical reason due to the scarcity of primary selective markers available Nicotiana tabacum

Cloned(Commentary)

Cloned (Comment) Organism
into vector pAST-IV containing a 1671 bp long modified version of the ASA2 gene without a putative transit peptide and with an ASA2 3'-non-coding region (204 bp) as the termination sequence. Expression cassette containing Prrn-ASA2 integrated into the region between accD and ycf4 of the tobacco plastome Nicotiana tabacum

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum
-
-
-

Synonyms

Synonyms Comment Organism
anthranilate synthase
-
 Nicotiana tabacum
ASA2
-
 Nicotiana tabacum

General Information

General Information Comment Organism
physiological function plastid transformed lines exhibit a higher level of anthranilate synthase activity that is less sensitive to tryptophan-feedback inhibition and, consequently, increases free tryptophan levels in leaves about 7fold. Overexpression of ASA2 gene does not result in any negative effects for the plants Nicotiana tabacum