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Literature summary for 4.1.3.27 extracted from

  • Ge, S.M.; Xie, B.E.; Chen, S.F.
    Characterization of two trpE genes encoding anthranilate synthase alpha-subunit in Azospirillum brasilense (2006), Biochem. Biophys. Res. Commun., 341, 494-499.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain JM109 Azospirillum brasilense

Protein Variants

Protein Variants Comment Organism
additional information the trpE-mutant is created by deletion of the fragment between positions +64 and +926 in the trpE coding region and replacement with the 1.2 kb Km resistance fragment from plasmid pUC4K, the mutant shows a marked inhibition by L-tryptophan compared to the wild type strain Azospirillum brasilense
additional information the trpE1(G)-mutant is created by deleting a DNA segment containing the N-terminal portion (from the 2nd codon to 374th codon) of the trpE1(G) gene by PCR amplification of chromosomal sequences flanking the trpE1(G) locus in wild type strain Yu62, the mutant shows no inhibition by L-tryptophan compared to the wild type strain Azospirillum brasilense

Inhibitors

Inhibitors Comment Organism Structure
L-tryptophan slightly inhibited Azospirillum brasilense

Organism

Organism UniProt Comment Textmining
Azospirillum brasilense
-
-
-
Azospirillum brasilense Yu62
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chorismate + L-Gln
-
Azospirillum brasilense anthranilate + pyruvate + L-glutamate
-
?
chorismate + L-Gln
-
Azospirillum brasilense Yu62 anthranilate + pyruvate + L-glutamate
-
?