Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain JM109 | Azospirillum brasilense |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the trpE-mutant is created by deletion of the fragment between positions +64 and +926 in the trpE coding region and replacement with the 1.2 kb Km resistance fragment from plasmid pUC4K, the mutant shows a marked inhibition by L-tryptophan compared to the wild type strain | Azospirillum brasilense |
additional information | the trpE1(G)-mutant is created by deleting a DNA segment containing the N-terminal portion (from the 2nd codon to 374th codon) of the trpE1(G) gene by PCR amplification of chromosomal sequences flanking the trpE1(G) locus in wild type strain Yu62, the mutant shows no inhibition by L-tryptophan compared to the wild type strain | Azospirillum brasilense |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-tryptophan | slightly inhibited | Azospirillum brasilense |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azospirillum brasilense | - |
- |
- |
Azospirillum brasilense Yu62 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chorismate + L-Gln | - |
Azospirillum brasilense | anthranilate + pyruvate + L-glutamate | - |
? | |
chorismate + L-Gln | - |
Azospirillum brasilense Yu62 | anthranilate + pyruvate + L-glutamate | - |
? |