Literature summary for 4.1.3.27 extracted from

Yamada, T.; Tozawa, Y.; Hasegawa, H.; Terakawa, T.; Ohkawa, Y.; Wakasa, K.
Use of a feedback-insensitive ? subunit of anthranilate synthase as a selectable marker for transformation of rice and potato. (2004), Mol. Breed., 14, 363-373.

Application

Application	Comment	Organism
molecular biology	use of the feedback-insensitive alpha-subunit OASA1D (N323D) of anthranilate synthase as a selectable marker for transformation of rice and potato, the selection system will prove applicable to a wide range of plant species and culture procedures	Oryza sativa

Cloned(Commentary)

Cloned (Comment)	Organism
cDNA(OASA1D) and the promoter of the maize ubiquitin gene is subcloned into pYT8C-Hm to generate pUASA1D, which also contains hpt as a selectable marker gene. For potato transformation, the vector p35SASA1D is constructed from pIG121-Hm, which contains the selectable marker genes hpt and nptII controlled by the 35S promoter of cauliflower mosaic virus, by replacing the beta-glucuronidase gene with the OASA1D cDNA	Oryza sativa

Cloned (Comment)

Organism

cDNA(OASA1D) and the promoter of the maize ubiquitin gene is subcloned into pYT8C-Hm to generate pUASA1D, which also contains hpt as a selectable marker gene. For potato transformation, the vector p35SASA1D is constructed from pIG121-Hm, which contains the selectable marker genes hpt and nptII controlled by the 35S promoter of cauliflower mosaic virus, by replacing the beta-glucuronidase gene with the OASA1D cDNA

Oryza sativa

Organism

Organism	UniProt	Comment	Textmining
Oryza sativa	-	-	-

Synonyms

Synonyms	Comment	Organism
OASA1D	feedback-insensitive alpha-subunit OASA1D (OASA1 with point mutation N323D)	Oryza sativa

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Release 2023.1 (January 2023)