Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Arctic Express (DE3) cells | Methylorubrum extorquens |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 0.1 M HEPES pH 7.5, 30%(w/v) PEG 400, 0.2 M MgCl2 | Methylorubrum extorquens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(2R)-4-malyl-CoA | competitive inhibitor | Methylorubrum extorquens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | dependent on | Methylorubrum extorquens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S)-4-malyl-CoA | Methylorubrum extorquens | - |
acetyl-CoA + glyoxylate | - |
r | |
acetyl-CoA + glyoxylate | Methylorubrum extorquens | - |
(2S)-4-malyl-CoA | - |
r | |
propionyl-CoA + glyoxylate | Methylorubrum extorquens | - |
(2S)-4-(beta-methyl)-malyl-CoA | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylorubrum extorquens | C5B113 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography and Superdex 200 gel filtration | Methylorubrum extorquens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S)-4-malyl-CoA | - |
Methylorubrum extorquens | acetyl-CoA + glyoxylate | - |
r | |
acetyl-CoA + glyoxylate | - |
Methylorubrum extorquens | (2S)-4-malyl-CoA | - |
r | |
propionyl-CoA + glyoxylate | - |
Methylorubrum extorquens | (2S)-4-(beta-methyl)-malyl-CoA | - |
r |
Subunits | Comment | Organism |
---|---|---|
homohexamer | 6 * 34000, calculated from amino acid sequence | Methylorubrum extorquens |
Synonyms | Comment | Organism |
---|---|---|
MCL | - |
Methylorubrum extorquens |