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Literature summary for 4.1.3.24 extracted from

  • Meister, M.; Saum, S.; Alber, B.E.; Fuchs, G.
    L-Malyl-coenzyme A/beta-methylmalyl-coenzyme A lyase is involved in acetate assimilation of the isocitrate lyase-negative bacterium Rhodobacter capsulatus (2005), J. Bacteriol., 187, 1415-1425.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.3.24

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Rhodobacter capsulatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
 malyl-CoA
-
 Rhodobacter capsulatus
0.021
-
 erythro-beta-methylmalyl-CoA
-
 Rhodobacter capsulatus
0.14
-
 acetyl-CoA
-
 Rhodobacter capsulatus
1.2
-
 glyoxylate
-
 Rhodobacter capsulatus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ stimulates Rhodobacter capsulatus
Mg2+ stimulates Rhodobacter capsulatus
Mn2+ stimulation by divalent ions, preference for Mn2+ Rhodobacter capsulatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 6 * 35000, SDS-PAGE Rhodobacter capsulatus
195000
-
 gel filtration Rhodobacter capsulatus

Organism

Organism UniProt Comment Textmining
Rhodobacter capsulatus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rhodobacter capsulatus

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:acetate-grown cell malyl-CoA lyase activity is 20fold upregulated in acetate-grown cells versus glucose-grown cells Rhodobacter capsulatus
-
culture condition:L-glucose-grown cell malyl-CoA lyase activity is 20fold upregulated in acetate-grown cells versus glucose-grown cells Rhodobacter capsulatus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.7
-
 cleavage of erythro-beta-methylmalyl-CoA Rhodobacter capsulatus
18
-
 cleavage of L-malyl-CoA Rhodobacter capsulatus
37
-
 condensation of acetyl-CoA with glyoxylate Rhodobacter capsulatus

Storage Stability

Storage Stability Organism
-20°C, 10% w/w glycerol, stable for several weeks Rhodobacter capsulatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + glyoxylate
-
 Rhodobacter capsulatus L-malyl-CoA
-
 r
propionyl-CoA + glyoxylate
-
 Rhodobacter capsulatus erythro-beta-methylmalyl-CoA
-
 r

Subunits

Subunits Comment Organism
hexamer 6 * 35000, SDS-PAGE Rhodobacter capsulatus

Synonyms

Synonyms Comment Organism
L-malyl-coenzyme A/beta-methylmalyl-coenzyme A lyase
-
 Rhodobacter capsulatus