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Literature summary for 4.1.3.24 extracted from

  • Herter, S.; Busch, A.; Fuchs, G.
    L-Malyl-coenzyme A lyase/beta-methylmalyl-coenzyme A lyase from Chloroflexus aurantiacus, a bifunctional enzyme involved in autotrophic CO(2) fixation (2002), J. Bacteriol., 184, 5999-6006.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.3.24

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Chloroflexus aurantiacus

Inhibitors

Inhibitors Comment Organism Structure
EDTA inhibits at 1 mM Chloroflexus aurantiacus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.061
-
 (3S)-3-carboxy-3-hydroxypropanoyl-CoA pH 7.1, 55°C Chloroflexus aurantiacus
0.089
-
 beta-methylmalyl-CoA pH 7.1, 55°C Chloroflexus aurantiacus
0.36
-
 acetyl-CoA pH 7.1, 55°C Chloroflexus aurantiacus
1.2
-
 propionyl-CoA pH 7.1, 55°C Chloroflexus aurantiacus
2
-
 glyoxylate pH 7.1, 55°C Chloroflexus aurantiacus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 4 mM, requirement Chloroflexus aurantiacus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 x * 38000, alpha5 or alpha6, SDS-PAGE Chloroflexus aurantiacus
210000
-
 gel filtration Chloroflexus aurantiacus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(3S)-3-carboxy-3-hydroxypropanoyl-CoA Chloroflexus aurantiacus
-
 acetyl-CoA + glyoxylate
-
 r

Organism

Organism UniProt Comment Textmining
Chloroflexus aurantiacus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Chloroflexus aurantiacus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.48
-
 acetyl-CoA condensation Chloroflexus aurantiacus
0.5
-
 propionyl-CoA condensation Chloroflexus aurantiacus
2.3
-
 L-malyl-CoA lyase activity Chloroflexus aurantiacus
6.5
-
 beta-methylmalyl-CoA lyase activity Chloroflexus aurantiacus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(3S)-3-carboxy-3-hydroxypropanoyl-CoA
-
 Chloroflexus aurantiacus acetyl-CoA + glyoxylate
-
 r
(3S)-3-carboxy-3-hydroxypropanoyl-CoA malyl-CoA Chloroflexus aurantiacus acetyl-CoA + glyoxylate
-
 r
propionyl-CoA + glyoxylate
-
 Chloroflexus aurantiacus erythro-beta-methylmalyl-CoA
-
 ?

Subunits

Subunits Comment Organism
? x * 38000, alpha5 or alpha6, SDS-PAGE Chloroflexus aurantiacus

Synonyms

Synonyms Comment Organism
L-malyl-CoA lyase/beta-methylmalyl-CoA lyase the two enzymes are identical and both enzymatic reactions are catalysed by one single bifunctional enzyme Chloroflexus aurantiacus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Chloroflexus aurantiacus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 stable for 20 min Chloroflexus aurantiacus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8.8
-
 (3S)-3-carboxy-3-hydroxypropanoyl-CoA pH 7.1, 55°C Chloroflexus aurantiacus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1
-
 at 55°C Chloroflexus aurantiacus