Protein Variants | Comment | Organism |
---|---|---|
DLCR6 (cimA::Gm) | citramalate synthase knockout mutant | Geobacter sulfurreducens |
DLCR7 (tdcB::Kn cimA::Gm) | double (ammonia-lyase (EC 4.3.1.19) and citramalate synthase) knockout mutant. DLCR5 (tdcB::Kn) a threonine ammonia-lyase knockout mutant (EC 4.3.1.19) is also constructed | Geobacter sulfurreducens |
additional information | strain DL1 used to construct strains. Construction of mutants via single-step gene replacement. Mutants lacking both enzymes (citramalate synthase and threonine ammonia-lyase) are auxotrophs for isoleucine, whereas single mutants are capable of growth in the absence of isoleucine | Geobacter sulfurreducens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetate + pyruvate | Geobacter sulfurreducens | citramalate synthase appears to have a high affinity for pyruvate, with 52.5% +/- 4.9% of the activity remaining when the pyruvate concentration is reduced from 1 mM to 0.1 mM | citramalate | - |
? | |
acetate + pyruvate | Geobacter sulfurreducens ATCC 51573 / DSM 12127 | citramalate synthase appears to have a high affinity for pyruvate, with 52.5% +/- 4.9% of the activity remaining when the pyruvate concentration is reduced from 1 mM to 0.1 mM | citramalate | - |
? | |
acetate + pyruvate | Geobacter sulfurreducens DSM 12127 | citramalate synthase appears to have a high affinity for pyruvate, with 52.5% +/- 4.9% of the activity remaining when the pyruvate concentration is reduced from 1 mM to 0.1 mM | citramalate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacter sulfurreducens | - |
- |
- |
Geobacter sulfurreducens ATCC 51573 / DSM 12127 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
enzymatic activity in mutant strain DLCR5 (tdcB::Kn), a threonine ammonia-lyase knockout mutant (EC 4.3.1.19), is 0.0051 micromol/min/mg | Geobacter sulfurreducens |
0.0004 | - |
in mutant strain DLCR6 (cimA::Gm) | Geobacter sulfurreducens |
0.0004 | - |
in mutant strain DLCR7 (tdcB::Kn cimA::Gm) | Geobacter sulfurreducens |
0.0074 | - |
DL1 (wild type) | Geobacter sulfurreducens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetate + pyruvate | citramalate synthase appears to have a high affinity for pyruvate, with 52.5% +/- 4.9% of the activity remaining when the pyruvate concentration is reduced from 1 mM to 0.1 mM | Geobacter sulfurreducens | citramalate | - |
? | |
acetate + pyruvate | citramalate synthase appears to have a high affinity for pyruvate, with 52.5% +/- 4.9% of the activity remaining when the pyruvate concentration is reduced from 1 mM to 0.1 mM | Geobacter sulfurreducens ATCC 51573 / DSM 12127 | citramalate | - |
? | |
acetate + pyruvate | citramalate synthase appears to have a high affinity for pyruvate, with 52.5% +/- 4.9% of the activity remaining when the pyruvate concentration is reduced from 1 mM to 0.1 mM | Geobacter sulfurreducens DSM 12127 | citramalate | - |
? | |
citramalate | - |
Geobacter sulfurreducens | 2-oxobutanoate + ? | - |
? | |
citramalate | - |
Geobacter sulfurreducens ATCC 51573 / DSM 12127 | 2-oxobutanoate + ? | - |
? | |
citramalate | - |
Geobacter sulfurreducens DSM 12127 | 2-oxobutanoate + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CimA | - |
Geobacter sulfurreducens |
citramalate synthase | - |
Geobacter sulfurreducens |
GSU1798 | represents a novel phylogenetic variant of the enzyme | Geobacter sulfurreducens |