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Literature summary for 4.1.3.22 extracted from

  • Risso, C.; Van Dien, S.J.; Orloff, A.; Lovley, D.R.; Coppi, M.V.
    Elucidation of an alternate isoleucine biosynthesis pathway in Geobacter sulfurreducens (2008), J. Bacteriol., 190, 2266-2274.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
DLCR6 (cimA::Gm) citramalate synthase knockout mutant Geobacter sulfurreducens
DLCR7 (tdcB::Kn cimA::Gm) double (ammonia-lyase (EC 4.3.1.19) and citramalate synthase) knockout mutant. DLCR5 (tdcB::Kn) a threonine ammonia-lyase knockout mutant (EC 4.3.1.19) is also constructed Geobacter sulfurreducens
additional information strain DL1 used to construct strains. Construction of mutants via single-step gene replacement. Mutants lacking both enzymes (citramalate synthase and threonine ammonia-lyase) are auxotrophs for isoleucine, whereas single mutants are capable of growth in the absence of isoleucine Geobacter sulfurreducens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetate + pyruvate Geobacter sulfurreducens citramalate synthase appears to have a high affinity for pyruvate, with 52.5% +/- 4.9% of the activity remaining when the pyruvate concentration is reduced from 1 mM to 0.1 mM citramalate
-
?
acetate + pyruvate Geobacter sulfurreducens ATCC 51573 / DSM 12127 citramalate synthase appears to have a high affinity for pyruvate, with 52.5% +/- 4.9% of the activity remaining when the pyruvate concentration is reduced from 1 mM to 0.1 mM citramalate
-
?
acetate + pyruvate Geobacter sulfurreducens DSM 12127 citramalate synthase appears to have a high affinity for pyruvate, with 52.5% +/- 4.9% of the activity remaining when the pyruvate concentration is reduced from 1 mM to 0.1 mM citramalate
-
?

Organism

Organism UniProt Comment Textmining
Geobacter sulfurreducens
-
-
-
Geobacter sulfurreducens ATCC 51573 / DSM 12127
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
enzymatic activity in mutant strain DLCR5 (tdcB::Kn), a threonine ammonia-lyase knockout mutant (EC 4.3.1.19), is 0.0051 micromol/min/mg Geobacter sulfurreducens
0.0004
-
in mutant strain DLCR6 (cimA::Gm) Geobacter sulfurreducens
0.0004
-
in mutant strain DLCR7 (tdcB::Kn cimA::Gm) Geobacter sulfurreducens
0.0074
-
DL1 (wild type) Geobacter sulfurreducens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetate + pyruvate citramalate synthase appears to have a high affinity for pyruvate, with 52.5% +/- 4.9% of the activity remaining when the pyruvate concentration is reduced from 1 mM to 0.1 mM Geobacter sulfurreducens citramalate
-
?
acetate + pyruvate citramalate synthase appears to have a high affinity for pyruvate, with 52.5% +/- 4.9% of the activity remaining when the pyruvate concentration is reduced from 1 mM to 0.1 mM Geobacter sulfurreducens ATCC 51573 / DSM 12127 citramalate
-
?
acetate + pyruvate citramalate synthase appears to have a high affinity for pyruvate, with 52.5% +/- 4.9% of the activity remaining when the pyruvate concentration is reduced from 1 mM to 0.1 mM Geobacter sulfurreducens DSM 12127 citramalate
-
?
citramalate
-
Geobacter sulfurreducens 2-oxobutanoate + ?
-
?
citramalate
-
Geobacter sulfurreducens ATCC 51573 / DSM 12127 2-oxobutanoate + ?
-
?
citramalate
-
Geobacter sulfurreducens DSM 12127 2-oxobutanoate + ?
-
?

Synonyms

Synonyms Comment Organism
CimA
-
Geobacter sulfurreducens
citramalate synthase
-
Geobacter sulfurreducens
GSU1798 represents a novel phylogenetic variant of the enzyme Geobacter sulfurreducens