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Literature summary for 4.1.3.16 extracted from
- 2-keto-4-Hydroxybutyrate aldolase. Identification as 2-keto-4-hydroxyglutarate aldolase, catalytic properties, and role in mammalian metabolism of L-homoserine (1971), Biochemistry, 10, 1353-1364.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-Ketobutyrate | competitive, Ki for 2-keto-4-hydroxybutyrate cleavage: 53 mM, Ki for 2-keto-4-hydroxyglutarate cleavage: 52 mM | Bos taurus |  |
| 2-Ketoglutarate | competitive, Ki for 2-keto-4-hydroxybutyrate cleavage: 18 mM, Ki for 2-keto-4-hydroxyglutarate cleavage: 18 mM | Bos taurus | |
| glycolaldehyde | competitive, Ki for 2-keto-4-hydroxybutyrate cleavage: 2.7 mM, Ki for 2-keto-4-hydroxyglutarate cleavage: 2.4 mM | Bos taurus | |
| NaBH4 | - |
Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
4-hydroxy-alpha-ketoglutarate | - |
Bos taurus | |
| 3.1 | - |
2-Keto-4-hydroxybutyrate | - |
Bos taurus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no requirement | Mammalia | |
| additional information | no requirement | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-Keto-4-hydroxybutyrate | Bos taurus | metabolism of L-homoserine | ? | - |
? | |
| 4-hydroxy-2-oxoglutarate | Mammalia | catabolism of hydroxyproline, condensation physiologically less important | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
bovine | - |
| Mammalia | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.53 | - |
2-keto-4-hydroxybutyrate | Bos taurus |
| 1.8 | - |
2-keto-4-hydroxyglutarate | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-Keto-4-hydroxybutyrate | D-isomer favoured | Bos taurus | Pyruvate + formaldehyde | - |
? | |
| 2-Keto-4-hydroxybutyrate | metabolism of L-homoserine | Bos taurus | ? | - |
? | |
| 4-Hydroxy-2-oxoglutarate | - |
Bos taurus | Pyruvate + glyoxylate | - |
? | |
| 4-hydroxy-2-oxoglutarate | catabolism of hydroxyproline, condensation physiologically less important | Mammalia | ? | - |
? | |
| additional information | mechanism | Bos taurus | ? | - |
? | |
| Pyruvate + formaldehyde | Keq: 4.1 mM | Bos taurus | 2-Keto-4-hydroxybutyrate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
enzyme assay at | Bos taurus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 59 | - |
constant decay of 0.011 1/min, slow inactivation | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.1 | - |
2-keto-4-hydroxybutyrate cleavage | Bos taurus |
| 8.8 | - |
2-keto-4-hydroxyglutarate cleavage | Bos taurus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9.5 | 2-keto-4-hydroxybutyrate cleavage | Bos taurus |
| 7 | 9 | 2-keto-4-hydroxyglutarate cleavage | Bos taurus |
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