Inhibitors | Comment | Organism | Structure |
---|---|---|---|
arsenite | - |
Rattus norvegicus | |
Cu2+ | 93% inhibition at 2 mM | Rattus norvegicus | |
cysteine | 65% inhibition at 10 mM | Rattus norvegicus | |
Hg2+ | complete inhibition at 2 mM | Rattus norvegicus | |
iodoacetate | 10% inhibition at 10 mM | Rattus norvegicus | |
N-ethylmaleimide | 64% inhibition at 10 mM | Rattus norvegicus | |
p-chloromercuribenzoate | complete inhibition at 10 mM | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.43 | - |
glyoxylate | - |
Rattus norvegicus | |
1.33 | - |
4-hydroxy-alpha-ketoglutarate | - |
Rattus norvegicus | |
10 | - |
pyruvate | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
rat | - |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | poor activity | Rattus norvegicus | - |
kidney | - |
Rattus norvegicus | - |
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.3 | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-Hydroxy-2-oxoglutarate | - |
Rattus norvegicus | Pyruvate + glyoxylate | - |
? | |
Glyoxylate + pyruvate | specific, codensation favoured, Keq: 0.73 mM | Rattus norvegicus | 4-Hydroxy-2-ketoglutarate | - |
? | |
additional information | specific for glyoxylate and pyruvate | Rattus norvegicus | ? | - |
? | |
additional information | equilibrium favours condensation, Keq: 0.73 1/mM | Rattus norvegicus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
enzyme assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | condensation, elimination | Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | - |
Rattus norvegicus |