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Literature summary for 4.1.2.9 extracted from

  • Petrareanu, G.; Balasu, M.C.; Zander, U.; Scheidig, A.J.; Szedlacsek, S.E.
    Preliminary X-ray crystallographic analysis of the D-xylulose 5-phosphate phosphoketolase from Lactococcus lactis (2010), Acta Crystallogr. Sect. F, 66, 805-807.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.2.9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) RIL cells Lactococcus lactis
expressed in Escherichia coli BL21(DE3) RIL cells Lactococcus lactis

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 17% (w/v) PEG 3350 and 0.2 M NaSCN, at 18°C Lactococcus lactis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ cofactor-bound Mg2+ is required for enzymatic activity Lactococcus lactis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
95727
-
 x * 95727, calculated from amino acid sequence Lactococcus lactis

Organism

Organism UniProt Comment Textmining
Lactococcus lactis
-
-
-
Lactococcus lactis
-
 subsp. lactis
-
Lactococcus lactis IL 1403
-
-
-
Lactococcus lactis IL 1403
-
 subsp. lactis
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap HP column chromatography and Superdex 200 gel filtration Lactococcus lactis
HisTrap Ni2+ metal affinity column chromatography and Superdex 200 gel filtration Lactococcus lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Fructose 6-phosphate + phosphate the enzyme has higher activity with D-xylulose 5-phosphate than D-fructose 6-phosphate Lactococcus lactis Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
 ?
D-Fructose 6-phosphate + phosphate the specificity constant for D-xylulose 5-phosphate is significantly higher than that for D-fructose 6-phosphate Lactococcus lactis Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
 ?
D-Fructose 6-phosphate + phosphate the enzyme has higher activity with D-xylulose 5-phosphate than D-fructose 6-phosphate Lactococcus lactis IL 1403 Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
 ?
D-Fructose 6-phosphate + phosphate the specificity constant for D-xylulose 5-phosphate is significantly higher than that for D-fructose 6-phosphate Lactococcus lactis IL 1403 Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
 ?
D-Xylulose 5-phosphate + phosphate the specificity constant for D-xylulose 5-phosphate is significantly higher than that for D-fructose 6-phosphate Lactococcus lactis Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
 ?
D-Xylulose 5-phosphate + phosphate the specificity constant for D-xylulose 5-phosphate is significantly higher than that for D-fructose 6-phosphate Lactococcus lactis IL 1403 Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
 ?
D-xylulose 5-phosphate + phosphate most specific substrate Lactococcus lactis acetyl phosphate + D-glyceraldehyde 3-phosphate
-
 ?
D-xylulose 5-phosphate + phosphate most specific substrate Lactococcus lactis IL 1403 acetyl phosphate + D-glyceraldehyde 3-phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 95727, calculated from amino acid sequence Lactococcus lactis

Synonyms

Synonyms Comment Organism
D-xylulose 5-phosphate phosphoketolase
-
 Lactococcus lactis

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate
-
 Lactococcus lactis
thiamine diphosphate dependent on Lactococcus lactis