Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.2.9 extracted from

  • Yevenes, A.; Frey, P.A.
    Cloning, expression, purification, cofactor requirements, and steady state kinetics of phosphoketolase-2 from Lactobacillus plantarum (2008), Bioorg. Chem., 36, 121-127.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
calcium thiamine diphosphate
-
Lactiplantibacillus plantarum
magnesium thiamine diphosphate
-
Lactiplantibacillus plantarum
manganese thiamine diphosphate
-
Lactiplantibacillus plantarum

Cloned(Commentary)

Cloned (Comment) Organism
into the vector pET21a+ for expression in Escherichia coli BL21DE3 cells Lactiplantibacillus plantarum
into the vectors pET21a+ and pET28b+ for expression in Escherichia coli BL21DE3 cells Lactiplantibacillus plantarum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0004
-
D-fructose 6-phosphate apparent Km value, Mn2+ Lactiplantibacillus plantarum
0.032
-
D-fructofuranose 6-phosphate apparent Km value, Mg2+ Lactiplantibacillus plantarum
0.16
-
D-fructose 6-phosphate apparent Km value, Ca2+ Lactiplantibacillus plantarum
2.9
-
phosphate D-fructose 6-phosphate and phosphate Lactiplantibacillus plantarum
3.6
-
D-xylulose 5-phosphate xylulose 5-phosphate and phosphate Lactiplantibacillus plantarum
7.5
-
phosphate xylulose 5-phosphate and phosphate Lactiplantibacillus plantarum
24
-
D-fructose 6-phosphate D-fructose 6-phosphate and phosphate Lactiplantibacillus plantarum

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Lactiplantibacillus plantarum
Mg2+
-
Lactiplantibacillus plantarum
Mn2+
-
Lactiplantibacillus plantarum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
SDS-PAGE Lactiplantibacillus plantarum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-fructose 6-phosphate + phosphate Lactiplantibacillus plantarum
-
?
-
?
D-Xylulose 5-phosphate + phosphate Lactiplantibacillus plantarum
-
Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
?

Organism

Organism UniProt Comment Textmining
Lactiplantibacillus plantarum Q88S87
-
-
Lactiplantibacillus plantarum Q88U67
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant phosphoketolase-2 is purified using Ni-NTA resin, a Biogel P6 and a Sephacryl-300 column, in a second purification approach a DEAE-Sepharose and a Sephadex-300 column are used Lactiplantibacillus plantarum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.052
-
purification step crude extract, histidine tail phosphoketolase-2 Lactiplantibacillus plantarum
0.2
-
purification step Biogel P6 column, histidine tail phosphoketolase-2 Lactiplantibacillus plantarum
0.61
-
purification step Sephacryl-300 column, histidine tail phosphoketolase-2 Lactiplantibacillus plantarum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose 6-phosphate + phosphate
-
Lactiplantibacillus plantarum ?
-
?
D-Xylulose 5-phosphate + phosphate
-
Lactiplantibacillus plantarum Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
?

Synonyms

Synonyms Comment Organism
phosphoketolase-1
-
Lactiplantibacillus plantarum
phosphoketolase-2
-
Lactiplantibacillus plantarum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
enzyme assay Lactiplantibacillus plantarum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
enzyme assay Lactiplantibacillus plantarum

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate
-
Lactiplantibacillus plantarum