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Literature summary for 4.1.2.9 extracted from

  • Whitworth, D.A.; Ratledge, C.
    Phosphoketolase in Rhodotorula graminis and other yeasts (1977), J. Gen. Microbiol., 102, 397-401.
No PubMed abstract available
Search for more literature for 4.1.2.9

Activating Compound

Activating Compound Comment Organism Structure
phosphate required Rhodotorula graminis
phosphate Km: 5.55 mM Rhodotorula graminis

Inhibitors

Inhibitors Comment Organism Structure
acetyl-CoA
-
 Rhodotorula graminis
ATP
-
 Rhodotorula graminis
citrate
-
 Rhodotorula graminis
dodecanoyl-CoA
-
 Rhodotorula graminis
NADH
-
 Rhodotorula graminis
NADPH
-
 Rhodotorula graminis
phosphoenolpyruvate
-
 Rhodotorula graminis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.25
-
 D-xylulose 5-phosphate
-
 Rhodotorula graminis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, Km: 0.000395 mM Rhodotorula graminis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rhodotorula graminis contribution of phosphoketolase to glucose catabolism is only slight ?
-
 ?

Organism

Organism UniProt Comment Textmining
Candida sp. (in: Saccharomycetales)
-
-
-
Candida sp. (in: Saccharomycetales) 107
-
-
-
Candida tropicalis
-
-
-
Rhodotorula glutinis
-
-
-
Rhodotorula graminis
-
-
-
Vanrija humicola
-
-
-

Oxidation Stability

Oxidation Stability Organism
phosphate is essential for stability Rhodotorula graminis

Purification (Commentary)

Purification (Comment) Organism
-
 Rhodotorula graminis

Storage Stability

Storage Stability Organism
4°C, 24 h, 50% loss of activity Rhodotorula graminis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose 6-phosphate + phosphate
-
 Vanrija humicola ?
-
 ?
D-Xylulose 5-phosphate + phosphate
-
 Rhodotorula glutinis Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
 ?
D-Xylulose 5-phosphate + phosphate
-
 Candida tropicalis Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
 ?
D-Xylulose 5-phosphate + phosphate
-
 Rhodotorula graminis Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
 ?
D-Xylulose 5-phosphate + phosphate
-
 Candida sp. (in: Saccharomycetales) Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
 ?
D-Xylulose 5-phosphate + phosphate
-
 Vanrija humicola Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
 ?
D-Xylulose 5-phosphate + phosphate
-
 Candida sp. (in: Saccharomycetales) 107 Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
 ?
additional information contribution of phosphoketolase to glucose catabolism is only slight Rhodotorula graminis ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Rhodotorula graminis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
27.5 32.5 27.5°C: about 70% of maximal activity, 32.5°C: about 30% of maximal activity Rhodotorula graminis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Rhodotorula graminis

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7 about 40% of maximal activity at pH 5.5 and at pH 7.0 Rhodotorula graminis

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate required Rhodotorula graminis
thiamine diphosphate Km: 0.000404 mM Rhodotorula graminis