Literature summary for 4.1.2.8 extracted from

Kulik, V.; Hartmann, E.; Weyand, M.; Frey, M.; Gierl, A.; Niks, D.; Dunn, M.F.; Schlichting, I.
On the structural basis of the catalytic mechanism and the regulation of the alpha subunit of tryptophan synthase from Salmonella typhimurium and BX1 from maize, two evolutionarily related enzymes (2005), J. Mol. Biol., 352, 608-620.

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Zea mays

Crystallization (Commentary)

Crystallization (Comment)	Organism
the crystal structure of BX1 suggusts that the faster catalytic rate of BX1 compared to the homologous alpha-subuni8t of tryptophan synthase EC 4.2.1.20, may due to a stabilzation of the active conformation, loop alphaL6 is closed and the catalytic glutamate, Glu134 is in the active conformation. There are two crystallographically independent molecules in the asymmetric unit of the rhombohedral BX1 crystal form	Zea mays

Crystallization (Comment)

Organism

the crystal structure of BX1 suggusts that the faster catalytic rate of BX1 compared to the homologous alpha-subuni8t of tryptophan synthase EC 4.2.1.20, may due to a stabilzation of the active conformation, loop alphaL6 is closed and the catalytic glutamate, Glu134 is in the active conformation. There are two crystallographically independent molecules in the asymmetric unit of the rhombohedral BX1 crystal form

Zea mays

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
plastid	-	Zea mays	9536	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
indole-3-glycerol phosphate	Zea mays	the indole is further converted to 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one, a secondary plant metabolite. BX1 cleaves indole-3-glycerol phosphate significantly faster to indole and glyceraldehyde 3-phosphate than the homologous alpha-subunit of tryptophan synthase, EC 4.2.1.20	indole + glyceraldehyde 3-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Zea mays	P42390	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Zea mays

Reaction

Reaction	Comment	Organism	Reaction ID
(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate = indole + D-glyceraldehyde 3-phosphate	the indole is further converted to 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one, a secondary plant metabolite. BX1 cleaves indole-3-glycerol phosphate significantly faster to indole and glyceraldehyde 3-phosphate than the homologous alpha-subunit of tryptophan synthase, EC 4.2.1.20. In the primary metabolism, indole diffuses through the connecting tunnel to the beta-active site where it is condensed with serine to form tryptophan and water	Zea mays	a

Source Tissue

Source Tissue	Comment	Organism	Textmining
seedling	-	Zea mays	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
indole-3-glycerol phosphate	-	Zea mays	indole + glyceraldehyde 3-phosphate	-	?
indole-3-glycerol phosphate	the indole is further converted to 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one, a secondary plant metabolite. BX1 cleaves indole-3-glycerol phosphate significantly faster to indole and glyceraldehyde 3-phosphate than the homologous alpha-subunit of tryptophan synthase, EC 4.2.1.20	Zea mays	indole + glyceraldehyde 3-phosphate	-	?

Synonyms

Synonyms	Comment	Organism
BX1	a homolog of the alpha-subunit of bifunctional trypthophan synthase	Zea mays